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Sample preparation for polar metabolites in bioanalysis
Analyst ( IF 3.6 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1039/c7an01333g Nicolas Drouin 1, 2, 3, 4, 5 , Serge Rudaz 1, 2, 3, 4, 5 , Julie Schappler 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1039/c7an01333g Nicolas Drouin 1, 2, 3, 4, 5 , Serge Rudaz 1, 2, 3, 4, 5 , Julie Schappler 1, 2, 3, 4, 5
Affiliation
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Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.
中文翻译：
生物分析中极性代谢物的样品制备
样品制备是任何生物分析工作流程的第一步，尤其是在代谢组学分析中，必须在不破坏分析仪器的情况下获得最大信息。由于其生物学意义，高极性代谢物，例如氨基酸，核碱基和儿茶酚胺，似乎在综合代谢组学分析领域引起了越来越多的兴趣，尽管它们从基质中的提取仍然是一个真正的挑战。在本文中，我们讨论了亲水性代谢物提取的实际做法和问题，包括改善分析前从复杂生物样品到干净提取物的相转移的新解决方案和观点。
更新日期：2017-11-27
中文翻译：
生物分析中极性代谢物的样品制备
样品制备是任何生物分析工作流程的第一步，尤其是在代谢组学分析中，必须在不破坏分析仪器的情况下获得最大信息。由于其生物学意义，高极性代谢物，例如氨基酸，核碱基和儿茶酚胺，似乎在综合代谢组学分析领域引起了越来越多的兴趣，尽管它们从基质中的提取仍然是一个真正的挑战。在本文中，我们讨论了亲水性代谢物提取的实际做法和问题，包括改善分析前从复杂生物样品到干净提取物的相转移的新解决方案和观点。
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