Hairpin loop-enhanced fluorescent copper nanoclusters and application in S1 nuclease detection†,Analyst

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Hairpin loop-enhanced fluorescent copper nanoclusters and application in S1 nuclease detection†
Analyst ( IF 3.6 ) Pub Date : 2017-11-27 00:00:00 , DOI: 10.1039/c7an01725a
Xian-sui Peng _{1,

2,

3,

4,

5} , Si-Yu Chen _{1,

2,

3,

4,

5} , Li-Juan Ou _{1,

2,

3,

4,

5} , Feng-Wu Luo _{1,

2,

3,

4,

5} , Si-Wen Qin _{1,

2,

3,

4,

5} , Ai-ming Sun _{1,

2,

3,

4,

5}

Affiliation

Novel highly fluorescent copper nanoclusters (CuNCs) were prepared by using 24 adenine–thymine pair dsDNA (AT24) with six-base (X6) loops (AT24-X6-hairpin DNA) as an effective template. The AT24 double strand stem serves as a template for CuNC formation, and the six-base sequence loop acts as specific regions to enhance the fluorescence intensity of CuNCs. Relative to the AT24-CuNCs, AT24-X6-hairpin CuNCs have greater fluorescence (5 times enhancement). What's more, the influence of the hairpin loop with different base types and base numbers on the fluorescence of CuNCs was first proposed and investigated. By choosing an AT24 double strand stem, any types of base loops can enhance the fluorescence of CuNCs. However, the fluorescence enhancement would be reduced with an increasing number of hairpin loop sequences. Besides this, the successful detection of S1 nuclease demonstrates its potential to be a new and robust fluorescent probe for sensing applications.

中文翻译：

发夹环增强型荧光铜纳米簇及其在S1核酸酶检测中的应用†

通过使用具有六碱基（X6）环（AT24-X6-发夹DNA）的24个腺嘌呤-胸腺嘧啶对dsDNA（AT24）作为有效模板，制备了新型的高荧光铜纳米簇（CuNC）。AT24双链茎充当CuNC形成的模板，六碱基序列环充当增强CuNCs荧光强度的特定区域。相对于AT24-CuNC，AT24-X6-发夹型CuNC具有更大的荧光（增强5倍）。此外，首先提出并研究了不同碱基类型和碱基编号的发夹环对CuNCs荧光的影响。通过选择AT24双链茎，任何类型的碱基环均可增强CuNCs的荧光。然而，随着发夹环序列数目的增加，荧光增强将降低。除此以外，

更新日期：2017-11-27

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