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Proteomic Characterization of Dermal Interstitial Fluid Extracted Using a Novel Microneedle-Assisted Technique
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-15 00:00:00 , DOI: 10.1021/acs.jproteome.7b00642 Bao Quoc Tran 1 , Philip R. Miller 2 , Robert M. Taylor 3 , Gabrielle Boyd 1 , Phillip M. Mach 1 , C. Nicole Rosenzweig 4 , Justin T. Baca 3 , Ronen Polsky 2 , Trevor Glaros 4
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-15 00:00:00 , DOI: 10.1021/acs.jproteome.7b00642 Bao Quoc Tran 1 , Philip R. Miller 2 , Robert M. Taylor 3 , Gabrielle Boyd 1 , Phillip M. Mach 1 , C. Nicole Rosenzweig 4 , Justin T. Baca 3 , Ronen Polsky 2 , Trevor Glaros 4
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As wearable fitness devices have gained commercial acceptance, interest in real-time monitoring of an individual’s physiological status using noninvasive techniques has grown. Microneedles have been proposed as a minimally invasive technique for sampling the dermal interstitial fluid (ISF) for clinical monitoring and diagnosis, but little is known about its composition. In this study, a novel microneedle array was used to collect dermal ISF from three healthy human donors and compared with matching serum and plasma samples. Using a shotgun quantitative proteomic approach, 407 proteins were quantified with at least one unique peptide, and of those, 135 proteins were differently expressed at least 2-fold. Collectively, these proteins tended to originate from the cytoplasm, membrane bound vesicles, and extracellular vesicular exosomes. Proteomic analysis confirmed previously published work that indicates that ISF is highly similar to both plasma and serum. In this study, less than one percent of proteins were uniquely identified in ISF. Taken together, ISF could serve as a minimally invasive alternative for blood-derived fluids with potential for real-time monitoring applications.
中文翻译：
新型微针辅助技术提取的皮肤间质液的蛋白质组学表征
随着可穿戴健身设备获得商业认可，使用无创技术实时监测个人生理状态的兴趣日益浓厚。已经提出将微针作为用于对皮肤间质液（ISF）进行采样以进行临床监测和诊断的微创技术，但对其组成知之甚少。在这项研究中，一种新型的微针阵列被用于从三个健康的人类供体中收集皮肤ISF，并将其与匹配的血清和血浆样品进行比较。使用a弹定量蛋白质组学方法，用至少一种独特的肽对407种蛋白质进行了定量，其中135种蛋白质的表达差异至少为2倍。总的来说，这些蛋白质倾向于起源于细胞质，膜结合囊泡和细胞外囊泡外泌体。蛋白质组学分析证实了先前发表的工作，该工作表明ISF与血浆和血清高度相似。在这项研究中，只有不到百分之一的蛋白质是在ISF中唯一鉴定的。综上所述，ISF可以作为血液来源液体的微创替代品，具有实时监测应用的潜力。
更新日期：2017-12-16
中文翻译：
新型微针辅助技术提取的皮肤间质液的蛋白质组学表征
随着可穿戴健身设备获得商业认可，使用无创技术实时监测个人生理状态的兴趣日益浓厚。已经提出将微针作为用于对皮肤间质液（ISF）进行采样以进行临床监测和诊断的微创技术，但对其组成知之甚少。在这项研究中，一种新型的微针阵列被用于从三个健康的人类供体中收集皮肤ISF，并将其与匹配的血清和血浆样品进行比较。使用a弹定量蛋白质组学方法，用至少一种独特的肽对407种蛋白质进行了定量，其中135种蛋白质的表达差异至少为2倍。总的来说，这些蛋白质倾向于起源于细胞质，膜结合囊泡和细胞外囊泡外泌体。蛋白质组学分析证实了先前发表的工作，该工作表明ISF与血浆和血清高度相似。在这项研究中，只有不到百分之一的蛋白质是在ISF中唯一鉴定的。综上所述，ISF可以作为血液来源液体的微创替代品，具有实时监测应用的潜力。
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