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A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury
Biomaterials ( IF 12.8 ) Pub Date : 2017-11-23 , DOI: 10.1016/j.biomaterials.2017.11.039
Xiaoyue Han , Rui Wang , Xinyu Song , Fabiao Yu , Changjun Lv , Lingxin Chen

The outburst of superoxide anion (O2) in mitochondrial during ischemia/reperfusion (I/R) process will cause a series of oxidative damage including polarity loss of mitochondrial membrane potential, overload of secondary cellular calcium, and cascade apoptosis. To monitor the O2radical dot level fluctuations as well as to evaluate the relationship between O2radical dot concentration and the degree of cell apoptosis during I/R process, we propose a ratiometric near-infrared mitochondrial targeting fluorescent probe Mito-Cy-Tfs for the detection of level changes of O2radical dot in cells and in vivo. The probe Mito-Cy-Tfs is composed of three moieties: near-infrared heptamethine cyanine as fluorescence signal transducer, trifluoromethanesulfonamide as fluorescence modulator, and lipophilic triphenylphosphonium cation as mitochondrial guider. The probe can well locate in mitochondria and respond the concentration changes of endogenous O2radical dot selectively and sensitively. The probe has been successfully utilized to image the endogenous O2radical dot fluctuations in four kinds of cell I/R models (glucose deprivation/reperfusion, serum deprivation/reperfusion, oxygen deprivation/reperfusion and glucose-serum-oxygen deprivation/reperfusion). The probe also exhibits deep tissue penetration for real-time imaging of O2radical dotconcentration in liver of I/R mice model. We confirm that the adoption of ischemic preconditioning (IPC) and postconditioning (IPTC) can protect liver from I/R injury. The probe can be employed to accurately indicate and evaluate the mutual relationship between the levels of O2radical dot and the degrees of organ damage during I/R, IPC and IPTC processes. The above applications make our new probe a potential candidate for the clinical surgery assessment.



中文翻译:

线粒体靶向近红外荧光探针用于生物成像和评估缺血/再灌注损伤期间内源性超氧阴离子的变化

超氧阴离子(O 2)在线粒体缺血/再灌注(I / R)过程中会导致一系列氧化损伤,包括线粒体膜电位的极性损失,继发性细胞钙超载和级联细胞凋亡。监视O 2激进点液位波动以及评估O 2之间的关系激进点在I / R过程中浓度和细胞凋亡程度,我们提出了一种比例式近红外线粒体靶向荧光探针Mito-Cy-Tfs,用于检测O 2的水平变化激进点在细胞和体内Mito-Cy-Tfs探针由三个部分组成:近红外七甲基花青作为荧光信号转导子,三氟甲磺酰胺作为荧光调节剂,亲脂性三苯基phosph阳离子作为线粒体引导子。该探针可以很好地定位于线粒体中并响应内源性O 2的浓度变化激进点有选择地和敏感地。该探针已成功地用于对内源性O 2进行成像激进点四种细胞I / R模型的波动(葡萄糖剥夺/再灌注,血清剥夺/再灌注,氧气剥夺/再灌注和葡萄糖-血清氧气剥夺/再灌注)。该探头还具有深层组织穿透性,可对O 2进行实时成像激进点 I / R小鼠模型在肝脏中的浓度。我们确认采用缺血预处理(IPC)和后处理(IPTC)可以保护肝脏免受I / R损伤。该探针可用于准确指示和评估O 2水平之间的相互关系激进点以及在I / R,IPC和IPTC过程中器官受损的程度。上述应用使我们的新探针成为临床手术评估的潜在候选者。

更新日期:2017-11-24
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