An ultrasensitive electrochemiluminescent d-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

An ultrasensitive electrochemiluminescent d-alanine biosensor based on the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-24 00:00:00 , DOI: 10.1039/c7ay02483e
Xia Lin _{1,

2,

3,

4,

5} , Shu Zhu _{1,

2,

3,

4,

5} , Qiao Xia _{1,

2,

3,

4,

5} , Jiao Ma _{1,

2,

3,

4,

5} , Yingzi Fu _{1,

2,

3,

4,

5}

Affiliation

Herein, an ultrasensitive and stereo-selective electrochemiluminescent (ECL) biosensor based on ECL signal amplification of luminol by the synergetic catalysis of a hemin-functionalized composite and gold–platinum nanowires (Au-PtNWs) has been designed for the detection of D-alanine (D-Ala). In the sensor design, D-amino acid oxidase (DAAO) was used to generate the ECL co-reactant (H₂O₂) for luminol in situ. The reduced graphene oxide-hemin-cysteine (rGO-H-Cys) composite and Au-PtNWs acted not only as amplification labels to amplify signals via synergetic catalysis, but also as an ideal nanocarrier to accelerate electron transfer. A great difference in ECL intensities toward D-Ala and L-Ala was obtained, and an obviously higher intensity was seen for D-Ala. Under optimal conditions, the ECL intensity had a linear relationship with the logarithm of the D-Ala concentration in the range from 1.0 × 10⁻⁹ to 5.0 × 10⁻³ M with a detection limit of 3.3 × 10⁻¹⁰ M (S/N = 3). This study provides a new method for the determination of D-Ala in food with high sensitivity, excellent selectivity, and good stability and reproducibility.

中文翻译：

超敏电化学发光的d-丙氨酸生物传感器，基于血红素功能化复合物和金-铂纳米线的协同催化

在此，超灵敏和立体选择性电化学发光（ECL）的生物传感器基于由的协同催化鲁米诺ECL信号放大一氯高铁血红素官能复合物和金-铂纳米线（金- PtNWs）已被设计用于检测的d丙氨酸（D -Ala）。在传感器设计中，D-氨基酸氧化酶（DAAO）用于原位生成鲁米诺的ECL共反应物（H ₂ O ₂）。还原的氧化石墨烯-血红素-半胱氨酸（rGO-H-Cys）复合材料和Au-PtNWs不仅用作扩增标记，还可以通过协同催化作用，也作为加速电子转移的理想纳米载体。得到的ECL强度对D -Ala和L -Ala的差异很大，并且D -Ala的强度明显更高。在最佳条件下，ECL强度与D- Ala浓度的对数在1.0×10 ^-9至5.0×10 ^-3 M范围内呈线性关系，检出限为3.3×10 ^-10 M（S / N = 3）。本研究提供了一种测定食品中D- Ala的新方法，具有高灵敏度，优异的选择性，良好的稳定性和可重复性。

更新日期：2017-11-24

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11