Sensitive and reliable paper-based glucose sensing mechanisms with smartphone readout using the L*a*b* color space,Analytical Methods

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Sensitive and reliable paper-based glucose sensing mechanisms with smartphone readout using the L*a*b* color space
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-08 00:00:00 , DOI: 10.1039/c7ay02124k
Deniz Baş _{1,

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Affiliation

Paper-based assay platforms play an important role among rapid test methods. It is essential to combine these promising platforms with smartphones which make on-site analysis possible without using external image processors. In this study, the performance of paper-based glucose assay methods was investigated by using the L*a*b* color space as the quantitative measurement. For this purpose, two glucose assay methods, namely quinone and iodine, were compared in terms of linearity, limit of detection (LOD) and reliability. A lower LOD and higher sensitivity were obtained by the quinone method while the iodine method may lead to large indeterminate errors due to the formation of non-uniform color distribution. Moreover, color measurement strategies were tested by using both computer software and mobile applications. For this purpose, scanned images were processed on a computer and the results were compared with smartphone based color measurements. The lowest LOD value of 0.38 mM glucose was obtained with the mobile application running on an Android operating system. Finally, glucose assay was performed on sour cherry nectar and honey and the results were compared with the spectrophotometric glucose assay. Using smartphones and the L*a*b* color space appears to be promising for reliable paper-based sensing as they are totally portable.

中文翻译：

基于L * a * b *色彩空间的智能手机读数，灵敏且可靠的基于纸张的葡萄糖感应机制

纸质化验平台在快速测试方法中起着重要作用。必须将这些有前途的平台与智能手机相结合，这样才能在不使用外部图像处理器的情况下进行现场分析。在这项研究中，使用L * a * b研究了纸基葡萄糖测定方法的性能*颜色空间作为定量度量。为此，在线性，检测限（LOD）和可靠性方面比较了两种葡萄糖测定方法，即醌和碘。通过醌法可获得较低的LOD和较高的灵敏度，而碘法可能会由于形成不均匀的颜色分布而导致较大的不确定误差。此外，通过使用计算机软件和移动应用程序对颜色测量策略进行了测试。为此，在计算机上处理扫描的图像，并将结果与基于智能手机的颜色测量结果进行比较。在Android操作系统上运行的移动应用程序获得的最低LOD值为0.38 mM葡萄糖。最后，对酸樱桃花蜜和蜂蜜进行葡萄糖测定，并将结果与分光光度葡萄糖测定法进行比较。使用智能手机和L * a * b *色彩空间似乎是可靠的基于纸张的传感技术，因为它们是完全便携式的。

更新日期：2017-11-24

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