Crambe abyssinica (crambe) is a new industrial oil crop that can grow on saline soil and tolerates salty water irrigation. Genetically engineered crambe in which the seed‐oil composition is manipulated for more erucic acid and less polyunsaturated fatty acid (PUFA) would be highly beneficial to industry. In this research, lysophosphatidic acid acyltransferase 2 RNA interference (CaLPAT2‐RNAi) was introduced into the crambe genome to manipulate its oil composition. The result showed in comparison with wild type, CaLPAT2‐RNAi could significantly reduce linoleic and linolenic acid content, simultaneously increasing erucic acid content. Systematic metabolism engineering was then carried out to further study CaLPAT2‐RNAi, combined with the overexpression of Brassica napus fatty acid elongase (BnFAE), Limnanthes douglasii LPAT (LdLPAT), and RNAi of endogenous fatty acid desaturase 2 (CaFAD2‐RNAi). Oil composition analysis on the tranformants' seeds showed that (a) with CaFAD2‐RNAi, PUFA content could be dramatically decreased, in comparison with BnFAE + LdLPAT + CaFAD2‐RNAi, and BnFAE + LdLPAT + CaFAD2‐RNAi + CaLPAT2‐RNAi seeds showed lower linolenic acid content; (b) BnFAE + LdLPAT + CaFAD2‐RNAi + CaLPAT2‐RNAi could increase the erucic acid content in crambe seed oil from less than 66.6% to 71.6%, whereas the highest erucic acid content of BnFAE + LdLPAT + CaFAD2‐RNAi was 79.2%; (c) although the four‐gene combination could not increase the erucic acid content of seed oil to a higher level than the others, it led to increased carbon resource deposited into C22:1 and C18:1 moieties and lower PUFA. Summarily, the present research indicates that suppression of LPAT2 is a new, promising strategy for seed‐oil biosynthesis pathway engineering, which would increase the value of crambe oil.

中文翻译：

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基因工程 Crambe abyssinica - 一种用于盐地改良的潜在高价值油料作物

Crambe abyssinica（克兰贝）是一种新的工业油料作物，可在盐渍土上生长，耐咸水灌溉。基因工程海葵，其中种子油成分被操纵以获得更多的芥酸和更少的多不饱和脂肪酸 (PUFA) 将对工业非常有益。在这项研究中，溶血磷脂酸酰基转移酶 2 RNA 干扰 (CaLPAT2-RNAi) 被引入海甘蓝基因组以操纵其油成分。结果表明，与野生型相比，CaLPAT2-RNAi 可显着降低亚油酸和亚麻酸含量，同时增加芥酸含量。然后进行系统代谢工程以进一步研究 CaLPAT2-RNAi，结合甘蓝型油菜脂肪酸延长酶 (BnFAE)、Limnanthes douglasii LPAT (LdLPAT)、和内源性脂肪酸去饱和酶 2 (CaFAD2-RNAi) 的 RNAi。转化子种子的油成分分析表明，(a) 与 BnFAE + LdLPAT + CaFAD2-RNAi 相比，CaFAD2-RNAi 的 PUFA 含量可以显着降低，并且 BnFAE + LdLPAT + CaFAD2-RNAi + CaLPAT2-RNAi 种子显示较低的亚麻酸含量；(b) BnFAE + LdLPAT + CaFAD2-RNAi + CaLPAT2-RNAi 可以将海葵籽油中的芥酸含量从低于 66.6% 提高到 71.6%，而 BnFAE + LdLPAT + CaFAD2-RNAi 的最高芥酸含量为 79.2% ; (c) 尽管四基因组合不能将种子油的芥酸含量提高到比其他组合更高的水平，但它导致沉积在 C22:1 和 C18:1 部分的碳资源增加并降低了 PUFA。总之，目前的研究表明抑制 LPAT2 是一种新的、