Two new nardosinone-type sesquiterpenoids, namely kanshone J (1) and kanshone K (2) along with seven known terpenoids (3–9) were isolated from the rhizomes and roots of Nardostachys jatamansi DC (Valerianaceae). The structures of these compounds were determined mainly by analysis of 1D-, 2D-NMR and MS data. In addition, the absolute configuration of compound 1 was assigned by application of the modified Mosher’s method. In an initial assay to evaluate their anti-neuroinflammatory effects, compounds 1–5 and 9 exhibited dose-dependent inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 cells, with IC₅₀ values ranging from 2.43 to 46.54 μM. Particularly, desoxo-narchinol A (3) and narchinol B (4) significantly inhibited LPS-induced NO overproduction in BV2 cells with IC₅₀ values of 3.48 ± 0.47 and 2.43 ± 0.23 μM, respectively. Furthermore, compounds 3 and 4 exhibited anti-neuroinflammatory effects by inhibiting the production of pro-inflammatory mediators, including prostaglandin E₂ (PGE₂), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) proteins, and pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF)-α, in LPS-stimulated BV2 and primary microglial cells.

从Nardostachys jatamansi DC（Valerianaceae）的根茎和根中分离出两个新的nardosinone型倍半萜，即kanshone J（1）和kanshone K（2）以及七个已知的萜类化合物（3 – 9）。这些化合物的结构主要通过1D-，2D-NMR和MS数据分析确定。另外，化合物1的绝对构型是通过应用改进的Mosher法确定的。在初始试验，以评估其抗神经炎效果，化合物1 - 5和9在BV2细胞中表现出对脂多糖（LPS）诱导的一氧化氮（NO）产生剂量依赖性抑制作用，IC ₅₀值为2.43至46.54μM。尤其是，去氧萘酚A（3）和萘酚B（4）显着抑制BV2细胞中LPS诱导的NO过量产生，其IC ₅₀值分别为3.48±0.47和2.43±0.23μM。此外，化合物3和4通过抑制促炎性介质（包括前列腺素E ₂（PGE ₂），诱导型一氧化氮合酶（iNOS）和环氧合酶2（COX-2）蛋白以及促炎性细胞因子，例如白介素（IL）-1β，IL-6和肿瘤坏死因子-α（TNF）- LPS刺激的BV2和原代小胶质细胞中的α。