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In vitro effects of endosulfan-based insecticides on mammalian sperm
Toxicology Research ( IF 2.2 ) Pub Date : 2017-11-23 00:00:00 , DOI: 10.1039/c7tx00251c M. C. Sánchez 1, 2, 3 , C. Alvarez Sedó 4, 5 , G. R. Chaufan 2, 3, 6 , M. Romanato 1, 2, 3 , R. Da Cuña 2, 3, 7, 8, 9 , F. Lo Nostro 2, 3, 7, 8, 9 , J. C. Calvo 1, 2, 3, 3, 10 , V. Fontana 1, 2, 3, 3, 10
Toxicology Research ( IF 2.2 ) Pub Date : 2017-11-23 00:00:00 , DOI: 10.1039/c7tx00251c M. C. Sánchez 1, 2, 3 , C. Alvarez Sedó 4, 5 , G. R. Chaufan 2, 3, 6 , M. Romanato 1, 2, 3 , R. Da Cuña 2, 3, 7, 8, 9 , F. Lo Nostro 2, 3, 7, 8, 9 , J. C. Calvo 1, 2, 3, 3, 10 , V. Fontana 1, 2, 3, 3, 10
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Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation.
中文翻译：
硫丹类杀虫剂对哺乳动物精子的体外作用
硫丹是一种有机氯化物杀虫剂，已在多个国家广泛用于保护农作物免受害虫侵害。正如数项研究表明，硫丹可以影响人类和动物的发育一样，本研究的目的是分析硫丹是否可以改变小鼠精子中的精子参数和染色质的缩合过程。来自附睾马尾的精子获自成熟的雄性小鼠，并在硫丹的两种市售制剂（CFs）（Master®和ZebraCiagro®）或单独的活性成分（AI）的存在下孵育。发现相对于对照，精子的运动性和生存力百分比显着降低。体外在谷胱甘肽和肝素存在下进行缩合反应。与AI，硫丹Master®和硫丹ZebraCiagro®孵育的精子显示出染色质的缩合增加。此外，TUNEL分析显示，与AI或对照组相比，当将精子与任一CF孵育时，DNA片段明显更高。精子细胞的超微结构分析显示，与硫丹AI或CFs孵育后，精子的血浆和顶体膜的结构发生了明显变化。这些结果表明，硫丹可以影响精子的完整性和体外染色质的缩聚以及DNA片段化。
更新日期：2017-11-23
中文翻译：
硫丹类杀虫剂对哺乳动物精子的体外作用
硫丹是一种有机氯化物杀虫剂，已在多个国家广泛用于保护农作物免受害虫侵害。正如数项研究表明，硫丹可以影响人类和动物的发育一样，本研究的目的是分析硫丹是否可以改变小鼠精子中的精子参数和染色质的缩合过程。来自附睾马尾的精子获自成熟的雄性小鼠，并在硫丹的两种市售制剂（CFs）（Master®和ZebraCiagro®）或单独的活性成分（AI）的存在下孵育。发现相对于对照，精子的运动性和生存力百分比显着降低。体外在谷胱甘肽和肝素存在下进行缩合反应。与AI，硫丹Master®和硫丹ZebraCiagro®孵育的精子显示出染色质的缩合增加。此外，TUNEL分析显示，与AI或对照组相比，当将精子与任一CF孵育时，DNA片段明显更高。精子细胞的超微结构分析显示，与硫丹AI或CFs孵育后，精子的血浆和顶体膜的结构发生了明显变化。这些结果表明，硫丹可以影响精子的完整性和体外染色质的缩聚以及DNA片段化。
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