N-glycosylation, as the most prevalent post-translational modification of proteins, plays an important part in many life processes. Characterization of N-glycome has been widely applied in the fields like biomarker mining and biopharmaceutical evaluation. However, the inherent low abundance of N-glycans hampers identification, structural elucidation and quantitation of the N-glycome using mass spectrometry (MS). Recently, a series of pre-treatment approaches, including N-glycan release, separation, enrichment and derivatization, are developed to achieve highly sensitive and accurate analysis of N-glycome. Herein, we summarize and highlight recent sample preparation strategies for MS-based identification and quantitation of N-glycome. We hope that this review serves as both an overview of the field and a guide to help scientists employing these powerful approaches in their research.

N-糖基化是蛋白质最普遍的翻译后修饰，在许多生命过程中都起着重要的作用。N-糖基的表征已广泛应用于生物标志物挖掘和生物药物评估等领域。然而，固有的低含量的N-聚糖妨碍了使用质谱（MS）进行N-糖基的鉴定，结构阐明和定量。最近，开发了一系列预处理方法，包括N-聚糖的释放，分离，富集和衍生化，以实现对N-糖基的高度灵敏和准确的分析。在这里，我们总结并重点介绍了基于MS的N-糖基的鉴定和定量的最新样品制备策略。