The development of a highly sensitive platform for multiplex circulating microRNAs (miRNAs) detection is important for clinical diagnosis. Here, a new type of porous hydrogel encapsulated photonic crystal (PhC) barcodes is presented with integrated rolling circle amplification (RCA) strategy for multiplex miRNA quantification. As the surrounding porous hydrogel shells of the PhC barcodes are interconnected inverse opal structure with hydrophilic scaffolds, they can provide homogeneous water surrounding for the miRNA targets reaction and RCA. The encapsulated PhC cores of the barcodes can offer stable diffraction peaks for encoding different miRNAs and their RCAs during the detection. By integrating the advantages of PhC barcodes and RCA, it is demonstrated that the technology shows acceptable accuracy and detection reproducibility for the rapid quantification of low‐abundance miRNAs, with the limits of detection of 20 fM. Thus, the proposed porous hydrogel encapsulated PhC barcodes provide a new platform for the multiplex quantification of low‐abundance targets for practical applications.

中文翻译：

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多孔水凝胶封装的光子条形码，用于多重MicroRNA定量

开发用于多重循环微RNA（miRNA）检测的高度敏感的平台对于临床诊断非常重要。在这里，提出了一种新型的多孔水凝胶封装的光子晶体（PhC）条形码，该条形码具有用于多重miRNA定量的集成滚环扩增（RCA）策略。由于PhC条形码​​的周围多孔水凝胶壳与亲水蛋白支架相互连接的蛋白石反结构，它们可以为miRNA靶标反应和RCA提供均匀的水。条码的封装PhC核可提供稳定的衍射峰，以在检测过程中编码不同的miRNA及其RCA。通过整合PhC条形码​​和RCA的优势，结果表明，该技术对低丰度miRNA的快速定量显示出可接受的准确性和检测重现性，检测限为20 fM。因此，提出的多孔水凝胶包封的PhC条码为实际应用中的低丰度目标的多重定量提供了一个新的平台。