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Protein-Based 3D Microstructures with Controllable Morphology and pH-Responsive Properties
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2017-11-22 00:00:00 , DOI: 10.1021/acsami.7b14915
Shuxin Wei 1 , Jie Liu 2 , Yuanyuan Zhao 3 , Tingbin Zhang 1 , Meiling Zheng 2, 4 , Feng Jin 2 , Xianzi Dong 2 , Jinfeng Xing 1 , Xuanming Duan 3
Affiliation  

The microtechnology of controlling stimuli-responsive biomaterials at micrometer scale is crucial for biomedical applications. Here, we report bovine serum albumin (BSA)-based three-dimensional (3D) microstructures with tunable surface morphology and pH-responsive properties via two-photon polymerization microfabrication technology. The laser processing parameters, including laser power, scanning speed, and layer distance, are optimized for the fabrication of well-defined 3D BSA microstructures. The tunable morphology of BSA microstructures and a wide range of pH response corresponding to the swelling ratio of 1.08–2.71 have been achieved. The swelling behavior of the microstructures can be strongly influenced by the concentration of BSA precursor, which has been illustrated by a reasonable mechanism. A panda face-shaped BSA microrelief with reversible pH-responsive properties is fabricated and exhibits unique “facial expression” variations in pH cycle. We further design a mesh sieve-shaped microstructure as a functional device for promising microparticle separation. The pore sizes of microstructures can be tuned by changing the pH values. Therefore, such protein-based microstructures with controllable morphology and pH-responsive properties have potential applications especially in biomedicine and biosensors.

中文翻译:

具有可控形态和pH响应特性的基于蛋白质的3D微结构

在微米尺度上控制刺激响应性生物材料的微技术对于生物医学应用至关重要。在这里,我们报告牛血清白蛋白(BSA)为基础的三维(3D)微结构,具有可调整的表面形态和pH响应特性,通过两光子聚合微制造技术。激光加工参数,包括激光功率,扫描速度和层距,已针对定义明确的3D BSA微结构的制造进行了优化。已经实现了BSA微观结构的可调节形态和广泛的pH响应,对应于1.08–2.71的溶胀率。微观结构的溶胀行为可能受到BSA前体浓度的强烈影响,这已通过合理的机理进行了说明。制成了具有可逆pH响应特性的熊猫脸形BSA微浮雕,并在pH循环中表现出独特的“面部表情”变化。我们进一步设计了筛孔筛状的微结构作为功能性设备,以实现有希望的微粒分离。可以通过改变pH值来调整微结构的孔径。因此,这种具有可控形态和pH响应特性的基于蛋白质的微结构具有潜在的应用,特别是在生物医学和生物传感器中。
更新日期:2017-11-23
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