Nucleophilic Water Capture or Proton Loss: Single Amino Acid Switch Converts δ-Cadinene Synthase into Germacradien-4-ol Synthase,ChemBioChem

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Nucleophilic Water Capture or Proton Loss: Single Amino Acid Switch Converts δ-Cadinene Synthase into Germacradien-4-ol Synthase
ChemBioChem ( IF 2.6 ) Pub Date : 2017-11-23 04:57:44 , DOI: 10.1002/cbic.201700531
Marianna Loizzi ₁ , Veronica González ₁ , David J. Miller ₁ , Rudolf K. Allemann ₁

Affiliation

Taking on water: Quenching of the final carbocation during terpene synthase catalysis, through proton loss or nucleophilic capture by water, is mediated by coordinated loop movements in the active site. A single amino acid switch in δ-cadinene synthase reduces hydrophobic contacts to allow water ingress during product release, resulting in an alcohol product.

中文翻译：

亲核性水捕获或质子损失：单个氨基酸开关将δ-卡丁烯合酶转化为Germacradien-4-ol合酶

吸收水：萜烯合酶催化过程中最终碳正离子的猝灭是通过质子损失或亲核性被水俘获，是由活性位点的协调环运动介导的。δ-cadinene合酶中的单个氨基酸开关可减少疏水性接触，从而在产品释放过程中使水进入，从而生成醇产品。

更新日期：2017-11-23

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