Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H2O2,Analytical Chemistry

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Endoplasmic Reticulum-Directed Ratiometric Fluorescent Probe for Quantitive Detection of Basal H2O2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-22 00:00:00 , DOI: 10.1021/acs.analchem.7b03809
Congcong Gao ₁ , Yong Tian ₁ , Rubo Zhang ₁ , Jing Jing ₁ , Xiaoling Zhang ₁

Affiliation

The endoplasmic reticulum (ER) has a central role in the fine-tuning of environmental and internal stimuli. We herein report a ratiometric fluorescent probe, α-Naph, capable of determining basal H₂O₂ in the ER. The probe specifically responds to H₂O₂. The limit of detection of the probe is as low as 38 nM, making it a feasible sensor to image intracellular basal H₂O₂. In addition, utilizing its ratiometric property, we are able to measure the concentration of H₂O₂ in the ER quantitatively, eliminating the error caused by the probe concentration and environment. The intracellular concentration of H₂O₂ in the ER is calculated to be 0.692 μM under normal conditions and 1.26 μM under the stimulation of phorbol myristate acetate.

中文翻译：

内质网定向比例荧光探针用于基础H ₂ O _2的定量检测

内质网（ER）在环境和内部刺激的微调中起着核心作用。我们在此报告了一种比例式荧光探针α-Naph，它能够确定ER中的基础H ₂ O ₂。探针对H ₂ O _2有特别反应。探针的检测限低至38 nM，使其成为对细胞内基础H ₂ O ₂成像的可行传感器。此外，利用其比例特性，我们能够定量测量ER中H ₂ O ₂的浓度，消除了由探针浓度和环境引起的误差。细胞内H ₂ O浓度在正常条件下，ER中的₂计算为0.692μM，在乙酸佛波醇肉豆蔻酸酯乙酸盐的刺激下，ER中的₂计算为1.26μM。

更新日期：2017-11-23

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