A novel single-cell analysis platform (SCA) was developed for the investigation of platelets adhesion to single human umbilical vein endothelial cell (HUVEC) via using the adhesion molecule (E-selectin) on the damaged HUVEC as the marker site, and integrating electrochemiluminescence (ECL) with the ultrasensitive [email protected] nanoprobes. The [email protected] nanocomposite, a kind of double layer zinc-coadsorbed carbon quantum dot (ZnCQDs) core-shell nanoprobe, was firstly constructed by using gold nanoparticles (AuNPs) as the core to load with ZnCQDs and then the citrate-modified silver nanoparticles (AgNPs) as the bridge to link AuNPs-ZnCQDs with ZnCQDs to form the core-shell with double layer ZnCQDs (DL-ZnCQDs) nanoprobe, revealed a 10-fold signal amplification. The H₂O₂-induced oxidative damage HUVECs were utilized as the cellular model on which anti-E-selectin functionalized nanoprobes specially recognized E-selectin, the SCA showed that the ECL signals decreased with platelets adhesion to single HUVEC. The proposed SCA could effectively and dynamically monitor the adhesion between single HUVEC and platelets in the absence and presence of collagen activation, moreover, be able to quantitatively detect the number of platelets adhesion to single HUVEC, and show a good analytical performance with linear range from 1 to 15 platelets. In contrast, the HUVEC was down-regulated the expression of adhesion molecules by treating with quercetin inhibitor, and the SCA also exhibited the feasibility for analysis of platelets adhesion to single HUVEC. Therefore, the single-cell analysis platform provided a novel and promising protocol for analysis of the single intercellular adhesion, and it will be beneficial to elucidate the pathogenesis of cardiovascular diseases.

开发了一种新颖的单细胞分析平台（SCA），用于通过使用受损的HUVEC上的粘附分子（E-选择素）作为标记位点并整合电化学发光来研究血小板与单个人脐静脉内皮细胞（HUVEC）的粘附（ECL）和超敏感的[受电子邮件保护的]纳米探针。[电子邮件保护]纳米复合材料是一种以锌纳米颗粒为核心的纳米碳复合物，首先以金纳米颗粒（AuNPs）为核心，负载ZnCQDs，然后以柠檬酸修饰的银为载体，构建了一种双层吸附锌的碳量子点（ZnCQDs）核壳纳米探针。纳米颗粒（AgNPs）作为连接AuNPs-ZnCQDs和ZnCQDs形成与双层ZnCQDs（DL-ZnCQDs）纳米探针的核壳的桥梁，显示出10倍的信号放大率。H ₂ O ₂诱导的氧化损伤HUVECs被用作抗E-选择素功能化的纳米探针特异识别E-选择素的细胞模型，SCA显示ECL信号随着血小板对单个HUVEC的粘附而降低。所提出的SCA可以有效和动态地监测单个HUVEC与血小板在不存在胶原蛋白活化的情况下的粘附力，此外，能够定量检测与单个HUVEC粘附的血小板数，并且在线性范围从1至15个血小板。相反，通过用槲皮素抑制剂处理，HUVEC下调了粘附分子的表达，并且SCA还显示出分析血小板对单个HUVEC粘附的可行性。所以，