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The development of a biomimetic enzyme-linked immunosorbent assay based on the molecular imprinting technique for the detection of enrofloxacin in animal-based food
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7ay02321a Jing Wang 1, 2, 3, 4 , Yaxin Sang 1, 2, 3, 4 , Weihua Liu 1, 2, 3, 4 , Na Liang 1, 2, 3, 4 , Xianghong Wang 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7ay02321a Jing Wang 1, 2, 3, 4 , Yaxin Sang 1, 2, 3, 4 , Weihua Liu 1, 2, 3, 4 , Na Liang 1, 2, 3, 4 , Xianghong Wang 1, 2, 3, 4, 5
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In this study, a direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method using molecularly imprinted polymers (MIPs) as artificial antibodies was developed to detect enrofloxacin (ENRO) in animal-based food. The MIPs were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, adsorption kinetic experiments and isothermal adsorption experiments. The results showed that molecularly imprinted polymers were successfully synthesized with a fast adsorption rate and high adsorption. Under the optimum conditions, the sensitivity (IC50) and limit of detection (IC15) of the established BELISA method were 85.7 ng mL−1 and 1.1 ng mL−1, respectively. The method was applied to the determination of ENRO in spiked beef and shrimp samples, and recoveries ranged from 74.4 to 86.8% with relative standard deviations (for n = 3) between 3.3 and 8.0%. The results correlated well with those obtained using ELISA and high performance liquid chromatography methods.
中文翻译：
基于分子印迹技术的仿生酶联免疫吸附法在动物食品中检测恩诺沙星的开发
在这项研究中，开发了一种直接竞争的仿生酶联免疫吸附测定（BELISA）方法，该方法使用分子印迹聚合物（MIP）作为人工抗体来检测动物性食品中的恩诺沙星（ENRO）。通过扫描电子显微镜（SEM），傅立叶变换红外光谱（FT-IR），吸附动力学实验和等温吸附实验对MIPs进行了表征。结果表明，成功地合成了分子印迹聚合物，具有高吸附速率和高吸附速率。在最佳条件下，已建立的BELISA方法的灵敏度（IC 50）和检测限（IC 15）为85.7 ng mL -1和1.1 ng mL -1， 分别。该方法用于测定加标牛肉和虾样品中的ENRO，回收率在74.4％至86.8％之间，相对标准偏差（n = 3）在3.3％至8.0％之间。结果与使用ELISA和高效液相色谱法获得的结果很好相关。
更新日期：2017-11-23
中文翻译：
基于分子印迹技术的仿生酶联免疫吸附法在动物食品中检测恩诺沙星的开发
在这项研究中，开发了一种直接竞争的仿生酶联免疫吸附测定（BELISA）方法，该方法使用分子印迹聚合物（MIP）作为人工抗体来检测动物性食品中的恩诺沙星（ENRO）。通过扫描电子显微镜（SEM），傅立叶变换红外光谱（FT-IR），吸附动力学实验和等温吸附实验对MIPs进行了表征。结果表明，成功地合成了分子印迹聚合物，具有高吸附速率和高吸附速率。在最佳条件下，已建立的BELISA方法的灵敏度（IC 50）和检测限（IC 15）为85.7 ng mL -1和1.1 ng mL -1， 分别。该方法用于测定加标牛肉和虾样品中的ENRO，回收率在74.4％至86.8％之间，相对标准偏差（n = 3）在3.3％至8.0％之间。结果与使用ELISA和高效液相色谱法获得的结果很好相关。
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