Fast and simple method for identification of adulteration of cow's milk with urea using diffuse reflectance spectroscopy,Analytical Methods

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Fast and simple method for identification of adulteration of cow's milk with urea using diffuse reflectance spectroscopy
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7ay02354e
Paulo R. A. B. de Toledo _{1,

2,

3,

4} , Aline T. Toci _{4,

5,

6,

7} , Helena R. Pezza _{1,

2,

3,

4} , Leonardo Pezza _{1,

2,

3,

4}

Affiliation

This work describes the development and application of a simple, fast, cheap, and clean method for the detection of adulteration of raw cow's milk by urea, using a combined spot test/diffuse reflectance spectroscopy procedure. The proposed method is based on the reaction of urea with a chromogenic reagent, p-dimethylaminobenzaldehyde (p-DAB) in acidified methanol, yielding a colored compound on the surface of a filter paper. The reaction parameters were optimized using chemometric experimental design. Reflectometric measurements of the colored product were carried out at 440 nm. The linear range was from 25 to 750 mg L⁻¹ (R = 0.992). The proposed method was successfully applied in the analysis of urea in cow's milk samples, demonstrating that it is a reliable alternative method for the screening and detection of urea in cow's milk.

中文翻译：

漫反射光谱法快速简便地鉴定尿素掺入牛奶的方法

这项工作描述了一种简单，快速，便宜和清洁的方法的开发和应用，该方法通过结合使用斑点测试/漫反射光谱法来检测尿素对生牛奶的掺假。所提出的方法基于尿素与生色试剂对-二甲基氨基苯甲醛（p -DAB）在酸化甲醇中的反应，从而在滤纸表面产生有色化合物。使用化学计量学实验设计优化了反应参数。对有色产物的反射测量在440nm下进行。线性范围为25至750 mg L ^-1（R= 0.992）。所提出的方法已成功应用于牛乳样品中尿素的分析，证明它是一种可靠的替代方法，可用于牛乳中尿素的筛查和检测。

更新日期：2017-11-23

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