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Colorimetric detection of hydrogen peroxide and glucose using brominated graphene
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7ay02212c Shikha Singh 1, 2, 3, 4, 5 , Kheyanath Mitra 1, 2, 3, 4, 5 , Rajshree Singh 1, 2, 3, 4, 5 , Archana Kumari 1, 2, 3, 4, 5 , Susanta Kumar Sen Gupta 1, 2, 3, 4, 5 , Nira Misra 4, 5, 6, 7 , Pralay Maiti 4, 5, 7, 8 , Biswajit Ray 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7ay02212c Shikha Singh 1, 2, 3, 4, 5 , Kheyanath Mitra 1, 2, 3, 4, 5 , Rajshree Singh 1, 2, 3, 4, 5 , Archana Kumari 1, 2, 3, 4, 5 , Susanta Kumar Sen Gupta 1, 2, 3, 4, 5 , Nira Misra 4, 5, 6, 7 , Pralay Maiti 4, 5, 7, 8 , Biswajit Ray 1, 2, 3, 4, 5
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Very recently, we have reported a novel peroxidase mimetic material, brominated graphene (GBR) having ∼3% bromine content, which, in combination with H2O2 and 3,3′,5,5′-tetramethylbenzidine (TMB), has shown the property of S2− ion recognition (Anal. Chem., 2017, 89, 783–791). In the present work, we further have investigated the kinetic assay and colorimetric sensing ability of GBR towards hydrogen peroxide (H2O2) and glucose. The Michaelis–Menten constants (Km) and maximum initial velocities (Vmax) of GBR have been found to be 10.98 mM and 3.60 × 10−8 M s−1, respectively, for H2O2 and 0.83 mM and 0.68 × 10−8 M s−1, respectively, for TMB. A sensor combining TMB and GBR has been fabricated, which, upon addition to H2O2 or glucose with glucose oxidase solution at pH 4.48, showed colorimetrically a significant increase in the oxidation of TMB. The fabricated sensor system has displayed linearity for H2O2 and glucose estimation in the range 0.50–5.00 mM and 40–100 mM, respectively, and the corresponding limits of detection are found to be 0.417 and 28.41 mM, respectively. The present sensor system is also highly reproducible and selective. The results of real samples using this colorimetric method have been found to be comparable with the conventional auto-analyser method.
中文翻译：
溴化石墨烯比色法检测过氧化氢和葡萄糖
最近，我们报道了一种新型的过氧化物酶模拟材料，溴含量约为3％的溴化石墨烯（GBR），与H 2 O 2和3,3'，5,5'-四甲基联苯胺（TMB）结合使用示S的属性2-离子识别（Anal.Chem.31：426-428中。，2017，89，783-791）。在本工作中，我们进一步研究了GBR对过氧化氢（H 2 O 2）和葡萄糖的动力学测定和比色传感能力。发现GBR的Michaelis-Menten常数（K m）和最大初始速度（V max）为10.98 mM和3.60×10 -8米每秒-1，分别用于h 2 ö 2和0.83 mM和0.68×10 -8米每秒-1，分别为TMB。已制造出结合了TMB和GBR的传感器，将其添加到pH值为4.48的H 2 O 2或葡萄糖氧化酶溶液中的葡萄糖中后，比色显示TMB的氧化显着增加。制成的传感器系统显示出H 2 O 2的线性葡萄糖估计值分别在0.50–5.00 mM和40–100 mM之间，相应的检出限分别为0.417和28.41 mM。本传感器系统也是高度可再现的和选择性的。已经发现使用这种比色法的真实样品的结果与常规的自动分析仪的结果是可比的。
更新日期：2017-11-23
中文翻译：
溴化石墨烯比色法检测过氧化氢和葡萄糖
最近，我们报道了一种新型的过氧化物酶模拟材料，溴含量约为3％的溴化石墨烯（GBR），与H 2 O 2和3,3'，5,5'-四甲基联苯胺（TMB）结合使用示S的属性2-离子识别（Anal.Chem.31：426-428中。，2017，89，783-791）。在本工作中，我们进一步研究了GBR对过氧化氢（H 2 O 2）和葡萄糖的动力学测定和比色传感能力。发现GBR的Michaelis-Menten常数（K m）和最大初始速度（V max）为10.98 mM和3.60×10 -8米每秒-1，分别用于h 2 ö 2和0.83 mM和0.68×10 -8米每秒-1，分别为TMB。已制造出结合了TMB和GBR的传感器，将其添加到pH值为4.48的H 2 O 2或葡萄糖氧化酶溶液中的葡萄糖中后，比色显示TMB的氧化显着增加。制成的传感器系统显示出H 2 O 2的线性葡萄糖估计值分别在0.50–5.00 mM和40–100 mM之间，相应的检出限分别为0.417和28.41 mM。本传感器系统也是高度可再现的和选择性的。已经发现使用这种比色法的真实样品的结果与常规的自动分析仪的结果是可比的。
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