Determination of malachite green, crystal violet, brilliant green and methylene blue by micro-cloud-point extraction and nonlinear laser wave-mixing detection interfaced to micellar capillary electrophoresis,Analytical Methods

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Determination of malachite green, crystal violet, brilliant green and methylene blue by micro-cloud-point extraction and nonlinear laser wave-mixing detection interfaced to micellar capillary electrophoresis
Analytical Methods ( IF 2.7 ) Pub Date : 2017-08-30 00:00:00 , DOI: 10.1039/c7ay01706e
Jean Sebastien Pradel _{1,

2,

3,

4} , William G. Tong _{1,

2,

3,

4}

Affiliation

A laser wave-mixing CE detection method is reported for separation and detection of malachite green (MG), crystal violet (CV), brilliant green (BG), methylene blue (MB), and the leuco-metabolites of MG and CV residues in aquacultures. This method applies a non-ionic surfactant for micro-cloud-point extraction (MCPE) followed by non-aqueous micellar capillary electrophoresis (MCE) for separation and nonlinear laser wave-mixing detection. The log–log plot of the MCE wave-mixing intensity vs. analyte concentration showed a good quadratic dependence with correlation coefficients greater than 0.989. The mass detection limits at an S/N of 2 were determined to be 2.27, 1.50, 23.8, 190, 27.61 and 22.2 attograms for MG, CV, BG, MB, leuco-MG, and leuco-CV, respectively. The concentration limits of detection (LODs) at an S/N of 2 were determined to be 4.13, 2.72, 10.2, 81.6, 50.2 and 40.3 pg mL⁻¹ for MG, CV, BG, MB, leuco-MG and leuco-CV, respectively. The intra-day variability (RSD) for the retention times ranges from 0.6 to 1.0% and the average inter-day variability (RSD) ranges from 0.7 to 4.7%. This method is simple, rapid and reliable, and it allows separation and simultaneous detection of MG, CV, MB, BG, and the leuco-metabolites of MG and CV in aquatic environments.

中文翻译：

通过微浊点萃取和胶束毛细管电泳连接的非线性激光波混合检测法测定孔雀石绿，结晶紫，亮绿和亚甲基蓝

报道了一种激光混合CE检测方法，用于分离和检测孔雀石绿（MG），结晶紫（CV），亮绿色（BG），亚甲基蓝（MB）以及MG和CV残留物中的无色代谢产物。水产养殖。该方法将非离子表面活性剂用于微浊点萃取（MCPE），然后使用非水胶束毛细管电泳（MCE）进行分离和非线性激光波混合检测。MCE混波强度与对数的对数对数图。分析物浓度表现出良好的二次相关性，相关系数大于0.989。对于MG，CV，BG，MB，无色MG和无色CV，在S / N为2时的质量检测限分别确定为2.27、1.50、23.8、190、27.61和22.2原子图。对于MG，CV，BG，MB，leuco-MG和leuco-CV，在S / N为2的检测浓度（LOD）被确定为4.13、2.72、10.2、81.6、50.2和40.3 pg mL ^-1，分别。保留时间的日内变异性（RSD）为0.6至1.0％，平均日间变异性（RSD）为0.7至4.7％。该方法简单，快速且可靠，可在水生环境中分离并同时检测MG，CV，MB，BG以及MG和CV的无色代谢产物。

更新日期：2017-11-23

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