An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis,Green Chemistry

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An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis
Green Chemistry ( IF 9.3 ) Pub Date : 2017-11-23 00:00:00 , DOI: 10.1039/c7gc02898a
Liaoyuan Zhang _{1,

2,

3,

4,

5} , Raushan Singh _{6,

7,

8,

9} , Sivakumar D _{6,

7,

8,

9} , Zewang Guo _{1,

2,

3,

4,

5} , Jiahuan Li _{1,

2,

3,

4,

5} , Fanbing Chen _{1,

2,

3,

4,

5} , Yuanzhi He _{1,

2,

3,

4,

5} , Xiong Guan _{1,

2,

3,

4,

5} , Yun Chan Kang _{9,

10,

11,

12} , Jung-Kul Lee _{6,

7,

8,

9}

Affiliation

Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

中文翻译：

利用无细胞多酶催化从乙醇生产乙醛，2,3-丁二醇和2-丁醇的人工合成途径

期望将乙醇升级为高级醇，但是使用当前的生物技术方法是困难的。在这项研究中，我们设计了一个完全人工的反应途径，可在乙醇脱氢酶，甲醛释放酶，2,3-丁二醇脱氢酶，二醇组成的无细胞生物系统中将乙醇升级为乙酰丙酮，2,3-丁二醇和2-丁醇。脱水酶和NADH氧化酶。在最佳条件下，从100 mM乙醇中分别获得理论收率的88.78％，2,3-丁二醇和2-丁醇。这些结果表明，这种人工合成途径是一种环境友好的新方法，可将生物乙醇升级为丙酮，2，3-丁二醇和2-丁醇。

更新日期：2018-01-02

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