An aptamer and functionalized nanoparticle-based strip biosensor for on-site detection of kanamycin in food samples,Analyst

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An aptamer and functionalized nanoparticle-based strip biosensor for on-site detection of kanamycin in food samples
Analyst ( IF 3.6 ) Pub Date : 2017-11-08 00:00:00 , DOI: 10.1039/c7an01476g
Jing Liu _{1,

2,

3,

4,

5} , Jingyi Zeng _{1,

2,

3,

4,

5} , Yaping Tian _{1,

2,

3,

4,

5} , Nandi Zhou _{1,

2,

3,

4,

5}

Affiliation

A lateral flow strip biosensor for fast, sensitive, low-cost and on-site detection of kanamycin was developed by using kanamycin-specific aptamer-modified gold nanoparticles (AuNPs-apt) as a probe and oligonucleotide DNA1-modified silver nanoparticles (AgNPs-DNA1) as a signal amplification element. Through the complementary sequences of DNA1 and the aptamer, the AgNP-DNA1-apt-AuNPs complex can be formed and further captured on the test zone of the strip, where a capture probe DNA2 complementary to the 3′-terminal of DNA1 was immobilized. In the presence of kanamycin, it can competitively bind to the aptamer, and then inhibit the formation of the complex and the accumulation of AuNPs on the test zone. AuNPs-apt can finally be captured on the control zone via the specific binding between biotin and streptavidin. The assay avoids multiple incubation and washing steps and can be completed within 10 min. By observing the color change of the test zone, a qualitative detection for kanamycin can be achieved by the naked eye, with the visual limit of 35 nM. Meanwhile, a linear detection range of 1–30 nM with a low detection limit of 0.0778 nM for quantitative analysis can be achieved by using a scanning reader. The lateral flow strip biosensor exhibited high specificity and stability. Moreover, it was applied to detect kanamycin in various food samples, indicating its great potential in field testing.

中文翻译：

适体和功能化的基于纳米颗粒的条型生物传感器，用于食品样品中卡那霉素的现场检测

通过使用卡那霉素特异性适体修饰的金纳米粒子（AuNPs-apt）作为探针和寡核苷酸DNA1修饰的银纳米粒子（AgNPs- DNA1）作为信号放大元件。通过DNA1和适体的互补序列，可以形成AgNP-DNA1-apt-AuNPs复合物，并进一步在条带的测试区捕获，在该条带上固定了与DNA1的3'-末端互补的捕获探针DNA2。在卡那霉素的存在下，它可以竞争性地与适体结合，然后抑制复合物的形成和AuNP在测试区的积累。最终可以通过控制区域捕获AuNPs-apt生物素和链霉亲和素之间的特异性结合。该测定避免了多个孵育和洗涤步骤，可以在10分钟内完成。通过观察测试区域的颜色变化，可以用肉眼定性检测卡那霉素，其视觉极限为35 nM。同时，使用扫描阅读器可以实现1–30 nM的线性检测范围和0.0778 nM的低检测限，以进行定量分析。横向流条生物传感器表现出高的特异性和稳定性。此外，它还用于检测各种食品样品中的卡那霉素，表明其在现场测试中具有巨大的潜力。

更新日期：2017-11-23

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