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Dynamic characterization of drug resistance and heterogeneity of the gastric cancer cell BGC823 using single-cell Raman spectroscopy
Analyst ( IF 3.6 ) Pub Date : 2017-11-07 00:00:00 , DOI: 10.1039/c7an01287j
Yong Zhang 1, 2, 3, 4, 5 , Ludi Jin 3, 4, 5, 6 , Jingjing Xu 3, 4, 5, 6 , Yuezhou Yu 3, 4, 5, 6 , Lin Shen 4, 5, 7, 8, 9 , Jing Gao 4, 5, 7, 8, 9 , Anpei Ye 1, 2, 3, 4, 5
Affiliation  

Drug resistance and heterogeneous characteristics of human gastric carcinoma cells (BGC823) under the treatment of paclitaxel (PTX) were investigated using single-cell Raman spectroscopy (RS). RS of normal and drug-resistant BGC823 cells (DR-BGC823) were collected and analyzed using arithmetic, statistic and individual spectrum analysis. The dynamic effects of paclitaxel (PTX) in normal and DR-BGC823 cells were evaluated dynamically. The RS intensity changed with PTX over time and produced distinct different results for the two types of cells. The average RS intensities of the normal BGC823 cells initially decreased and then increased under PTX treatment after 24 hours. In contrast, upon exposure to PTX, the average intensity of the DR-BGC823 cells initially increased within 12 hours and then gradually decreased and approached a steady state. The temporal variation of the typical component in the cells was analyzed by comparing the ratios between Raman bands. More importantly, the heterogeneous characteristics of the BGC823 cells under PTX treatment were quantified and clustered using hierarchical trees combined with RS intensity changes. The ‘outlier’ cells related to drug resistance were discriminated. The heterogeneity of the normal BGC823 cells under drug treatment gradually appeared over time, and was evaluated with the eigenvalues of principal component analysis (PCA). Our study indicates that single-cell RS may be useful in systematically and dynamically characterizing the drug response of cancer cells at the single-cell level.

中文翻译:

使用单细胞拉曼光谱法动态表征胃癌细胞BGC823的耐药性和异质性

使用单细胞拉曼光谱法(RS)研究了紫杉醇(PTX)处理下人胃癌细胞(BGC823)的耐药性和异质性。收集正常和耐药BGC823细胞(RS-BGC823)的RS,并使用算术,统计和单个光谱分析进行分析。紫杉醇(PTX)在正常和DR-BGC823细胞中的动态效果进行了动态评估。RS强度随PTX随时间变化,并且对于两种类型的细胞产生明显不同的结果。正常BGC823细胞的平均RS强度开始下降,然后在24小时后经PTX处理后增加。相反,在暴露于PTX后,DR-BGC823细胞的平均强度最初在12小时内增加,然后逐渐降低并达到稳定状态。通过比较拉曼谱带之间的比率,分析了细胞中典型成分的时间变化。更重要的是,使用分层树结合RS强度变化,对PTX处理的​​BGC823细胞的异质特征进行了量化和聚类。区分了与耐药有关的“异常”细胞。随着时间的推移,正常BGC823细胞的异质性逐渐出现,并通过主成分分析(PCA)的特征值进行评估。我们的研究表明,单细胞RS可能在系统性和动态地表征单细胞水平上癌细胞的药物反应中有用。利用分层树结合RS强度变化,对PTX处理后的BGC823细胞的异质性特征进行定量和聚类。区分了与耐药有关的“异常”细胞。随着时间的推移,正常BGC823细胞的异质性逐渐出现,并通过主成分分析(PCA)的特征值进行评估。我们的研究表明,单细胞RS可能在系统性和动态地表征单细胞水平上癌细胞的药物反应中有用。利用分层树结合RS强度变化,对PTX处理后的BGC823细胞的异质性特征进行定量和聚类。区分了与耐药有关的“异常”细胞。随着时间的推移,正常BGC823细胞的异质性逐渐出现,并通过主成分分析(PCA)的特征值进行评估。我们的研究表明,单细胞RS可能在系统性和动态地表征单细胞水平上癌细胞的药物反应中有用。随着时间的推移,正常BGC823细胞的异质性逐渐出现,并通过主成分分析(PCA)的特征值进行评估。我们的研究表明,单细胞RS可能在系统性和动态地表征单细胞水平上癌细胞的药物反应中有用。随着时间的推移,正常BGC823细胞的异质性逐渐出现,并通过主成分分析(PCA)的特征值进行评估。我们的研究表明,单细胞RS可能在系统性和动态地表征单细胞水平上癌细胞的药物反应中有用。
更新日期:2017-11-22
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