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An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization
Analyst ( IF 3.6 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7an01244f Ying-Xu Chen 1, 2, 3, 4, 5 , Wen-Jing Zhang 1, 2, 3, 4, 5 , Ke-Jing Huang 1, 2, 3, 4, 5 , Mingbo Zheng 4, 6, 7, 8 , Ya-Cen Mao 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7an01244f Ying-Xu Chen 1, 2, 3, 4, 5 , Wen-Jing Zhang 1, 2, 3, 4, 5 , Ke-Jing Huang 1, 2, 3, 4, 5 , Mingbo Zheng 4, 6, 7, 8 , Ya-Cen Mao 1, 2, 3, 4, 5
Affiliation
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In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA–RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe2) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin–horseradish peroxidase (HRP) conjugates to catalyze the H2O2 + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe2 nanosheets, the competitive hybridization configuration and the signal amplification of the H2O2 + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001–100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis.
中文翻译：
基于二硒化钨纳米片和竞争性RNA-RNA杂交的电化学microRNA传感平台
在这项工作中，我们报告了使用竞争性RNA-RNA杂交配置的用于microRNA-21（miRNA-21）检测的超灵敏电化学生物传感器。将与靶标miRNA具有相同序列的生物素化miRNA与样品混合，并与靶标miRNA竞争固定在二硒化钨（WSe 2）纳米片修饰电极上的巯基化RNA探针。此后，通过与链霉亲和素-辣根过氧化物酶（HRP）偶联物形成杂交的生物素化miRNA，以催化H 2 O 2 +对苯二酚（HQ）系统，可获得电流响应。受益于WSe 2的高比表面积纳米片，竞争性杂交配置和H 2 O 2 + HQ检测系统的信号放大，所提出的测定方法对目标miRNA的线性范围为0.0001–100 pM，检测极限为0.06 fM（S / N = 3 ），并且对碱基错配的miRNA序列显示出出色的辨别能力。因此，设计的平台在生物医学研究和早期临床诊断中对miRNA的检测具有广阔的前景。
更新日期：2017-11-22
中文翻译：
基于二硒化钨纳米片和竞争性RNA-RNA杂交的电化学microRNA传感平台
在这项工作中，我们报告了使用竞争性RNA-RNA杂交配置的用于microRNA-21（miRNA-21）检测的超灵敏电化学生物传感器。将与靶标miRNA具有相同序列的生物素化miRNA与样品混合，并与靶标miRNA竞争固定在二硒化钨（WSe 2）纳米片修饰电极上的巯基化RNA探针。此后，通过与链霉亲和素-辣根过氧化物酶（HRP）偶联物形成杂交的生物素化miRNA，以催化H 2 O 2 +对苯二酚（HQ）系统，可获得电流响应。受益于WSe 2的高比表面积纳米片，竞争性杂交配置和H 2 O 2 + HQ检测系统的信号放大，所提出的测定方法对目标miRNA的线性范围为0.0001–100 pM，检测极限为0.06 fM（S / N = 3 ），并且对碱基错配的miRNA序列显示出出色的辨别能力。因此，设计的平台在生物医学研究和早期临床诊断中对miRNA的检测具有广阔的前景。
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