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Facile immobilization of DNA using an enzymatic his-tag mimic
Chemical Communications ( IF 4.3 ) Pub Date : 2017-11-22 00:00:00 , DOI: 10.1039/c7cc07207d Pascal Röthlisberger 1, 2, 3, 4, 5 , Fabienne Levi-Acobas 1, 2, 3, 4, 5 , Ivo Sarac 1, 2, 3, 4, 5 , Bruno Baron 3, 6, 7, 8 , Patrick England 3, 6, 7, 8 , Philippe Marlière 9, 10, 11, 12, 13 , Piet Herdewijn 14, 15, 16, 17, 18 , Marcel Hollenstein 1, 2, 3, 4, 5
Chemical Communications ( IF 4.3 ) Pub Date : 2017-11-22 00:00:00 , DOI: 10.1039/c7cc07207d Pascal Röthlisberger 1, 2, 3, 4, 5 , Fabienne Levi-Acobas 1, 2, 3, 4, 5 , Ivo Sarac 1, 2, 3, 4, 5 , Bruno Baron 3, 6, 7, 8 , Patrick England 3, 6, 7, 8 , Philippe Marlière 9, 10, 11, 12, 13 , Piet Herdewijn 14, 15, 16, 17, 18 , Marcel Hollenstein 1, 2, 3, 4, 5
Affiliation
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Methods for immobilization of DNA on solid supports are in high demand. Herein, we present a generally applicable enzymatic method for the immobilization of DNA without any prior chemical derivatization. This strategy relies on the homopolymerization of the modified triphosphate dImTP by the TdT. The resulting enzymatic his-tag mimic ensures binding of DNA on Ni-NTA agarose. The usefulness of this method is highlighted by the immobilization of functional nucleic acids without impairing their specific activities.
中文翻译：
使用酶促His-tag模拟物轻松固定DNA
迫切需要将DNA固定在固体支持物上的方法。在本文中，我们提出了一种普遍适用的酶促方法，无需任何事先的化学衍生化即可固定化DNA。该策略依赖于改性的三磷酸dImTP通过TdT的均聚。所得的酶促组氨酸标签模拟物确保了DNA在Ni-NTA琼脂糖上的结合。通过固定功能性核酸而不损害其比活性，突出了该方法的有用性。
更新日期：2017-12-05
中文翻译：
使用酶促His-tag模拟物轻松固定DNA
迫切需要将DNA固定在固体支持物上的方法。在本文中，我们提出了一种普遍适用的酶促方法，无需任何事先的化学衍生化即可固定化DNA。该策略依赖于改性的三磷酸dImTP通过TdT的均聚。所得的酶促组氨酸标签模拟物确保了DNA在Ni-NTA琼脂糖上的结合。通过固定功能性核酸而不损害其比活性，突出了该方法的有用性。
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