Given the increasing interest in alternative processes for producing biodiesel, we focused on the latest screening of lipases and bioprocess design using multiple feedstocks. The implementation of lipase technology to the biodiesel industry is in the early stages. However, current research has made phenomenal advances in generating lipase variants and in engineering biodiesel production. The structural insights into lipase stability, together with primary screening, have opened up opportunities for acquiring lipase variants that are highly tolerant under industrially relevant conditions. The versatility of lipases is promising for process intensification, where time-consuming and costly steps can possibly be avoided. To judiciously overcome uncertainties in the biodiesel industry, further research on technology development integrated with supply chain models is necessary.

鉴于人们对生产生物柴油的替代方法的兴趣日益浓厚，我们专注于脂肪酶的最新筛选和使用多种原料的生物工艺设计。脂肪酶技术在生物柴油行业中的实施尚处于初期阶段。然而，当前的研究在产生脂肪酶变体和工程生物柴油生产方面取得了惊人的进步。对脂肪酶稳定性的结构见解，以及初步的筛选，为获得在工业上相关条件下具有高度耐受性的脂肪酶变体提供了机会。脂肪酶的多功能性对于增强工艺很有希望，因为可以避免耗时且昂贵的步骤。为了明智地克服生物柴油行业的不确定性，