Present communication reports the effect of amphiphilic matrices and nano-clay platelets on the aggregation properties of a water soluble cationic fluorescent dye Nile Blue Chloride (NBC) in Langmuir-Blodgett (LB) films. In-situ Brewster Angle Microscopic (BAM) studies showed distinct domain structures of complex and hybrid Langmuir monolayer at the air-water interface. UV-vis absorption spectra showed non-fluorescent H-dimeric band in concentrated aqueous solution of NBC and in complex LB film of NBC with stearic acid. By changing various parameters, a great control over H-dimeric states has been achieved in clay incorporated hybrid LB films. These films can act as efficient fluorescence probe.

本通讯报道了两亲性基质和纳米粘土血小板对Langmuir-Blodgett（LB）膜中水溶性阳离子荧光染料尼罗蓝氯化物（NBC）聚集特性的影响。原位布鲁斯特角显微镜（BAM）研究表明，复杂的混合兰格缪尔单层在气-水界面处具有明显的畴结构。紫外可见吸收光谱显示，在NBC的浓水溶液和NBC与硬脂酸的复合LB膜中，无荧光的H-二聚体谱带。通过改变各种参数，在掺入粘土的混合LB膜中已经实现了对H-二聚体状态的良好控制。这些膜可以用作有效的荧光探针。