The hierarchy of human hemopoietic progenitor cells that produce lymphoid and granulocytic–monocytic (myeloid) lineages is unclear. Multiple progenitor populations produce lymphoid and myeloid cells, but they remain incompletely characterized. Here we demonstrated that lympho-myeloid progenitor populations in cord blood — lymphoid-primed multi-potential progenitors (LMPPs), granulocyte-macrophage progenitors (GMPs) and multi-lymphoid progenitors (MLPs) — were functionally and transcriptionally distinct and heterogeneous at the clonal level, with progenitors of many different functional potentials present. Although most progenitors had the potential to develop into only one mature cell type (‘uni-lineage potential’), bi- and rarer multi-lineage progenitors were present among LMPPs, GMPs and MLPs. Those findings, coupled with single-cell expression analyses, suggest that a continuum of progenitors execute lymphoid and myeloid differentiation, rather than only uni-lineage progenitors’ being present downstream of stem cells.

中文翻译：

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单细胞分析揭示了人类淋巴样髓系祖细胞的连续性

尚不清楚产生淋巴样和粒细胞-单核（髓样）谱系的人类造血祖细胞的层次。多个祖细胞群会产生淋巴样和髓样细胞，但它们的特征仍不完全。在这里，我们证明了脐带血中的淋巴-髓样祖细胞（淋巴样多潜能祖细胞（LMPPs），粒细胞巨噬细胞祖细胞（GMPs）和多淋巴祖细胞（MLPs））在克隆功能上和转录上是不同的和异质的水平，具有许多不同功能潜能的祖细胞。尽管大多数祖细胞都有发展为仅一种成熟细胞类型的潜能（“单谱系潜能”），但LMPP，GMP和MLP中仍存在双谱系和罕见的多谱系祖细胞。这些发现，