Brettanomyces is a yeast species responsible for wine and cider spoilage, producing volatile phenols that result in off-odors and loss of fruity sensorial qualities. Current commercial detection methods for these spoilage species are liable to frequent false positives, long culture times and fungal contamination. In this work, an interdigitated (IDE) biosensor was created to detect Brettanomyces using immunological reactions and impedance spectroscopy analysis. To promote efficient antibody immobilization on the electrodes’ surface and to decrease non-specific adsorption, a Self-Assembled Monolayer (SAM) was developed. An impedance spectroscopy analysis, over four yeast strains, confirmed our device's increased efficacy. Compared to label-free sensors, antibody biosensors showed a higher relative impedance. The results also suggested that these biosensors could be a promising method to monitor some spoilage yeasts, offering an efficient alternative to the laborious and expensive traditional methods.

酒香酵母是导致葡萄酒和苹果酒变质的酵母，会产生挥发性酚，导致异味和水果感官品质下降。对于这些变质物质的当前商业检测方法容易导致频繁的假阳性，长的培养时间和真菌污染。在这项工作中，创建了一个叉指型（IDE）生物传感器来检测布雷塔酵母菌使用免疫反应和阻抗谱分析。为了促进有效的抗体固定在电极表面并减少非特异性吸附，开发了自组装单分子膜（SAM）。对四种酵母菌株进行的阻抗谱分析证实了我们设备的功效提高。与无标记传感器相比，抗体生物传感器显示出更高的相对阻抗。结果还表明，这些生物传感器可能是监测某些腐败酵母的有前途的方法，为费力且昂贵的传统方法提供了有效的替代方法。