Influence of the Carotenoid Composition on the Conformational Dynamics of Photosynthetic Light-Harvesting Complexes,The Journal of Physical Chemistry Letters

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Influence of the Carotenoid Composition on the Conformational Dynamics of Photosynthetic Light-Harvesting Complexes
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2017-11-21 00:00:00 , DOI: 10.1021/acs.jpclett.7b02634
Marijonas Tutkus ₁ , Jevgenij Chmeliov _{1,

2} , Danielis Rutkauskas ₁ , Alexander V. Ruban ₃ , Leonas Valkunas _{1,

2}

Affiliation

Nonphotochemical quenching (NPQ) is the major self-regulatory mechanism of green plants, performed on a molecular level to protect them from an overexcitation during the direct sunlight. It is believed that NPQ becomes available due to conformational dynamics of the light-harvesting photosynthetic complexes and involves a direct participation of carotenoids. In this work, we perform a single-molecule microscopy on major light-harvesting complexes (LHCII) from different Arabidopsis thaliana mutants exhibiting various carotenoid composition. We show how the distinct carotenoids affect the dynamics of the conformational switching between multiple coexisting light-emitting states of LHCII and demonstrate that properties of the quenched conformation are not influenced by the particular carotenoids available in LHCII. We also discuss the possible origin of different conformational states and relate them to the fluorescence decay kinetics observed during the bulk measurements.

中文翻译：

类胡萝卜素成分对光合光吸收复合物构象动力学的影响

非光化学猝灭（NPQ）是绿色植物的主要自我调节机制，在分子水平上执行以保护植物免受阳光直射期间的过度激发。据信，由于光捕获光合复合物的构象动力学，NPQ变得可用，并且涉及类胡萝卜素的直接参与。在这项工作中，我们对来自不同拟南芥的主要采光复合物（LHCII）进行了单分子显微镜检查突变体表现出各种类胡萝卜素组成。我们展示了不同的类胡萝卜素如何影响LHCII的多个共存发光状态之间构象转换的动力学，并证明了淬灭构象的性质不受LHCII中可用的特定类胡萝卜素的影响。我们还讨论了不同构象态的可能起源，并将它们与在批量测量过程中观察到的荧光衰减动力学联系起来。

更新日期：2017-11-22

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