Rapid identification of health threatening bacteria and/or spores present in small concentration in sample fluids is of utmost importance. Efficient sample preparation and molecular detection aims to achieving this goal. Two processes must be conducted successively: the concentration of the targets in a small volume with simultaneous purification, followed by their lysis to provide accessible DNA templates. Conventional PCR is then used in situ to identify the targets.

In this work we present an original approach combining an efficient concentration and purification of the bacteria and spores, a rapid and efficient grinding lysis step, working even for polluted samples, and the integration of the process in a semi-automated device. The method is very efficient and rapid: it can concentrate and detect less than 10 targets in 1 mL of sample, even if the sample is contaminated by some environmental contaminants. The most resistant spores are successfully lysed.

In this study, we successively present the principle and performances of the method, and its integration on a in a semi-automated device. Perspectives to fully integrated system are discussed.

快速鉴定样品液中浓度低的危害健康的细菌和/或孢子至关重要。高效的样品制备和分子检测旨在实现这一目标。必须连续进行两个过程：在同时纯化的情况下以小体积浓缩靶标，然后将其裂解以提供可及的DNA模板。然后就地使用常规PCR来鉴定靶标。

在这项工作中，我们提出了一种原始方法，该方法结合了细菌和孢子的有效浓缩和纯化，快速有效的研磨裂解步骤，甚至适用于污染样品的方法以及将过程集成到半自动化设备中的方法。该方法非常高效且快速：即使样品被某些环境污染物污染，它也可以在1 mL样品中浓缩并检测不到10个目标。最抗性的孢子已成功裂解。

在这项研究中，我们相继介绍了该方法的原理和性能，以及在半自动化设备中的集成。讨论了对完全集成系统的观点。