In the present work, a biosensor adapted in a 96-well microplate has been coupled with a smartphone-based photometer in order to develop a low-cost colorimetric multi-sample dispositive. The strength of this biosensing system is based on the integration of the biosensor into the 96-well microplate and the use of a smartphone and free image analysis software as a microplate reader. The performance of the proposed biosensor has been demonstrated to determine glucose in several human serum samples. This method is simple, cost-effective, sensitive and selective for the determination of glucose in serum, with detection limits of 1.8 mg/dL and a good linearity over the range 6–88 mg/dL. Precision was also satisfactory (relative standard deviation, %RSD<5). The behaviour of the biosensing system has also been compared with the conventional derivatization method, adding all the reagents in solution and measuring the absorption at 653 nm. Results obtained indicated that this approach simplifies significantly the analytical measurements, avoids the need to prepare derivatization reagents, is portable and allows multiple in-situ measurements simultaneously. Thus, the simplicity of the test has been improved not only in the reaction step but also in the response measurement.

在当前的工作中，已将适用于96孔微孔板的生物传感器与基于智能手机的光度计结合使用，以开发低成本的比色多样品沉积剂。这种生物传感系统的优势在于将生物传感器集成到96孔微孔板中，以及使用智能手机和免费的图像分析软件作为微孔板读取器。已经证明了所提出的生物传感器的性能可以测定几种人血清样品中的葡萄糖。该方法简单，经济高效，灵敏且选择性高，可测定血清中的葡萄糖，检出限为1.8 mg / dL，线性范围为6–88 mg / dL。精度也令人满意（相对标准偏差，％RSD <5）。还已将生物传感系统的行为与常规衍生化方法进行了比较，将所有试剂添加到溶液中并测量653 nm处的吸收。获得的结果表明，该方法大大简化了分析测量，避免了制备衍生试剂的需要，便于携带，并允许同时进行多个原位测量。因此，不仅在反应步骤中而且在响应测量中都改善了测试的简便性。