Comprehensive Mass Spectrometric Survey of Streptococcus pyogenes Subcellular Proteomes,Journal of Proteome Research

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Comprehensive Mass Spectrometric Survey of Streptococcus pyogenes Subcellular Proteomes
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2017-12-04 00:00:00 , DOI: 10.1021/acs.jproteome.7b00701
Laura Wilk ₁ , Lotta Happonen ₁ , Johan Malmström ₁ , Heiko Herwald ₁

Affiliation

Streptococcus pyogenes is a major global health burden causing a wide variety of diseases. Because a vaccine against this bacterium is still lacking, vaccine candidates or antimicrobial therapies are urgently needed. Here we use an invasive and clinically relevant streptococcal M1 serotype to characterize the bacterial proteome in-depth. An elaborate fractionation technique is employed to separate the different cell fractions, followed by shotgun mass-spectrometry analysis, allowing us to confirm the expression of nearly two-thirds (1022) of the 1690 open reading frames predicted for the streptococcal M1 reference proteome. In contrast with other studies, we present the entire isolated membrane proteome, which opens up a whole new source for drug targets. We show both the unique and most prevalent proteins for each cellular fraction and analyze the presence of predicted cell-wall-anchored proteins and lipoproteins. With our approach, we also identify a variety of novel proteins whose presence has not been reported in previous proteome studies. Proteins of interest, potential virulence factors, and drug or vaccine targets are discussed for each cellular fraction. Overall, the results of this work represent the so-far widest proteomic approach to characterize the protein composition and localization in S. pyogenes.

中文翻译：

化脓性链球菌亚细胞蛋白质组的综合质谱研究

化脓性链球菌是造成各种疾病的主要全球健康负担。由于仍然缺乏针对这种细菌的疫苗，因此迫切需要候选疫苗或抗菌疗法。在这里，我们使用侵袭性和临床相关链球菌M1血清型来深入表征细菌蛋白质组。精细的分级分离技术可用于分离不同的细胞碎片，然后进行shot弹枪质谱分析，从而使我们能够确定针对链球菌M1参考蛋白质组预测的1690个开放阅读框中近三分之二（1022）的表达。与其他研究相反，我们介绍了整个分离的膜蛋白质组，这为药物靶标打开了一个全新的来源。我们展示了每个细胞部分的独特和最流行的蛋白质，并分析了预测的细胞壁锚定蛋白质和脂蛋白的存在。通过我们的方法，我们还可以鉴定出各种新型蛋白质，这些蛋白质的存在在以前的蛋白质组研究中尚未得到报道。讨论了每个细胞部分的目标蛋白质，潜在的毒力因子以及药物或疫苗靶标。总的来说，这项工作的结果代表了迄今为止最广泛的蛋白质组学方法，用于表征蛋白质的组成和定位。化脓性链球菌。

更新日期：2017-12-05

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