Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling,Analyst

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Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling
Analyst ( IF 3.6 ) Pub Date : 2017-11-06 00:00:00 , DOI: 10.1039/c7an01423f
Henson L. Lee Yu _{1,

2,

3,

4} , Yinghua Zhang _{2,

3,

4,

5} , I-Ming Hsing _{1,

2,

3,

4,

5}

Affiliation

One of the promising approaches to address the challenge of detecting dilute nucleic acid analytes is exonuclease III-aided target recycling. In this strategy, the target DNA self-assembles with the reactant DNA probes and displays itself as a reactant and product at the same time. This provides an autonomous mechanism to release and reuse the analyte from each round of reactions for repetitive cycles, which amplifies the signal without amplifying the analyte itself. However, for very low amounts of the analyte, it takes a considerably long time before a detectable signal is generated. Thus, in this paper, we report a kinetically-enhanced target recycling strategy by designing two more target recycling sub-reactions that are triggered by the byproducts of the first reaction involving the target analyte. In this manner, concentrations of up to 0.5 pM of target DNA can be detected in 15 minutes.

中文翻译：

通过多次通过核酸外切酶III辅助的靶标回收来增强运动力的DNA检测

解决核酸稀释分析物挑战的一种有前途的方法是核酸外切酶III辅助的靶标回收。在这种策略中，目标DNA与反应物DNA探针自组装，并同时显示为反应物和产物。这提供了一种自主机制，可以在每个循环的反应循环中释放和重新使用分析物，以进行重复循环，从而在不放大分析物本身的情况下放大了信号。但是，对于极少量的分析物，在生成可检测信号之前要花费相当长的时间。因此，在本文中，我们通过设计另外两个目标回收子反应来报告动力学增强的目标回收策略，该反应由涉及目标分析物的第一次反应的副产物触发。以这种方式，浓度最高为0。

更新日期：2017-11-21

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