Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing substrates featuring the same configuration. Here, a set of deoxygenated cyclophellitols are presented which enable inter-class profiling of β-glucosidases and β-galactosidases.

中文翻译：

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面向基于广谱活性的糖苷酶探针：脱氧环磷脂醇氮丙啶的合成和评估

基于活动的蛋白质谱分析已成为可视化复杂生物样品中糖苷酶的强大工具。作为具有相同构型的糖苷酶加工底物的探针，几种构型的环磷脂醇异构体已显示出高选择性。在此，提出了一组脱氧的环糖醇，它们能够进行β-葡萄糖苷酶和β-半乳糖苷酶的类间分析。