Modifying RNA through either splicing or editing is a fundamental biological process for creating protein diversity from the same genetic code. Developing novel chemical biology tools for RNA editing has potential to transiently edit genes and to provide a better understanding of RNA biochemistry. Current techniques used to modify RNA include the use of ribozymes, adenosine deaminase, and tRNA endonucleases. Herein, we report a nanozyme that is capable of splicing virtually any RNA stem–loop. This nanozyme is comprised of a gold nanoparticle functionalized with three enzymes: two catalytic DNA strands with ribonuclease function and an RNA ligase. The nanozyme cleaves and then ligates RNA targets, performing a splicing reaction that is akin to the function of the spliceosome. Our results show that the three-enzyme reaction can remove a 19 nt segment from a 67 nt RNA loop with up to 66% efficiency. The complete nanozyme can perform the same splice reaction at 10% efficiency. These splicing nanozymes represent a new promising approach for gene manipulation that has potential for applications in living cells.

中文翻译：

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位点选择性 RNA 剪接纳米酶：金纳米颗粒上的 DNAzyme 和 RtcB 缀合物

通过剪接或编辑修饰 RNA 是从相同遗传密码创造蛋白质多样性的基本生物过程。开发用于 RNA 编辑的新型化学生物学工具有可能瞬时编辑基因并提供对 RNA 生物化学的更好理解。目前用于修饰 RNA 的技术包括使用核酶、腺苷脱氨酶和 tRNA 核酸内切酶。在此，我们报道了一种能够拼接几乎任何 RNA 茎环的纳米酶。这种纳米酶由金纳米颗粒组成，并用三种酶进行功能化：两条具有核糖核酸酶功能的催化 DNA 链和一种 RNA 连接酶。纳米酶切割并连接 RNA 靶标，执行类似于剪接体功能的剪接反应。我们的结果表明，三酶反应可以从 67 nt RNA 环中去除 19 nt 片段，效率高达 66%。完整的纳米酶可以以 10% 的效率进行相同的剪接反应。这些剪接纳米酶代表了一种新的有前途的基因操作方法，具有在活细胞中应用的潜力。