Synthetic Oligosaccharide Libraries and Microarray Technology: A Powerful Combination for the Success of Current Glycosaminoglycan Interactomics,ChemMedChem

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Synthetic Oligosaccharide Libraries and Microarray Technology: A Powerful Combination for the Success of Current Glycosaminoglycan Interactomics
ChemMedChem ( IF 3.6 ) Pub Date : 2017-12-06 , DOI: 10.1002/cmdc.201700620
Vitor H Pomin ₁ , Xu Wang ₂

Affiliation

Glycosaminoglycans (GAGs) are extracellular matrix and/or cell‐surface sulfated glycans crucial to the regulation of various signaling proteins, the functions of which are essential in many pathophysiological systems. Because structural heterogeneity is high in GAG chains and purification is difficult, the use of structurally defined GAG oligosaccharides from natural sources as molecular models in both biophysical and pharmacological assays is limited. To overcome this obstacle, GAG‐like oligosaccharides of well‐defined structures are currently being synthesized by chemical and/or enzymatic means in many research groups around the world. These synthetic GAG oligosaccharides serve as useful molecular tools in studies of GAG–protein interactions. In this review, besides discussing the commonest routes used for the synthesis of GAG oligosaccharides, we also survey some libraries of these synthetic models currently available for research and discuss their activities in interaction studies with functional proteins, especially through the microarray approach.

中文翻译：

合成寡糖文库和微阵列技术：当前糖胺聚糖相互作用组学成功的强大组合

糖胺聚糖 (GAG) 是细胞外基质和/或细胞表面硫酸化聚糖，对于调节各种信号蛋白至关重要，其功能在许多病理生理系统中至关重要。由于 GAG 链结构异质性高且纯化困难，因此在生物物理和药理学测定中使用天然来源的结构明确的 GAG 寡糖作为分子模型受到限制。为了克服这一障碍，世界各地的许多研究小组目前正在通过化学和/或酶促手段合成结构明确的 GAG 类寡糖。这些合成的 GAG 寡糖可作为 GAG-蛋白质相互作用研究中有用的分子工具。在这篇综述中，除了讨论用于合成 GAG 寡糖的最常见途径外，我们还调查了目前可用于研究的这些合成模型的一些库，并讨论了它们在与功能蛋白相互作用研究中的活动，特别是通过微阵列方法。

更新日期：2017-12-06

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