Single nucleotide polymorphism (SNP) in lipoprotein lipase (LPL) gene (rs1801177) is strongly associated with the increased progression of atherosclerosis, threatening global public health. In this work, a relatively simple, specific and ultrasensitive electrochemical DNA biosensor was constructed to detect rs1801177 for the first time. A glass carbon electrode was modified with fullerene (C₆₀)/polyamidoamine (PAMAM)/gold (Au) nanoparticles nanocomposites film. In addition the nitrogen-doped graphene (N-G)/palladium platinum (PdPt) bimetallic nanoparticle/ polyaniline (PANI) nanohybrids were synthesised and used to label the signal probes. These nanohybrids have abundant active groups, and efficient redox and catalytic activity, allowing them to be used as the nanocarrier for a redox nanoprobe without the additional modification of electroactive substance and catalyst, which could effectively simplify the operation procedure and shorten the analysis time. With the catalysis of H₂O₂ by nanohybrids, the detection signal of N-G/PdPt/PANI itself could be significantly enhanced, lead to the improvement of the sensitivity. Under optimal conditions, the electrochemical DNA biosensor exhibited desirable performance for the determination of rs1801177 with a wide linearity ranging from 10 fM to 10 nM and a relatively low detection limit of 3.33 fM (S/N=3). The proposed biosensor showed excellent selectivity to the target DNA compared to possible interfering substances. The results suggested that this method has potential applications in clinical research.

脂蛋白脂肪酶（LPL）基因（rs1801177）中的单核苷酸多态性（SNP）与动脉粥样硬化进展的增加密切相关，威胁全球公共健康。在这项工作中，构建了一个相对简单，特异且超灵敏的电化学DNA生物传感器来首次检测rs1801177。玻璃碳电极用富勒烯（C ₆₀）/聚酰胺酸（PAMAM）/金（Au）纳米颗粒纳米复合材料薄膜。此外，还合成了氮掺杂的石墨烯（NG）/钯铂（PdPt）双金属纳米颗粒/聚苯​​胺（PANI）纳米杂化物，并用于标记信号探针。这些纳米杂化物具有丰富的活性基团，并且具有高效的氧化还原和催化活性，使其无需额外修饰电活性物质和催化剂即可用作氧化还原纳米探针的纳米载体，从而可以有效简化操作程序并缩短分析时间。随着H ₂ O ₂的催化通过纳米杂交技术，NG / PdPt / PANI自身的检测信号可以得到显着增强，从而提高了灵敏度。在最佳条件下，电化学DNA生物传感器表现出理想的测定rs1801177的性能，其线性范围为10 fM至10 nM，检测限相对较低，为3.33 fM（S / N = 3）。与可能的干扰物质相比，拟议的生物传感器对目标DNA表现出极好的选择性。结果表明该方法在临床研究中具有潜在的应用前景。