In this manuscript, a disposable paper-based analytical device comprised of a closed bipolar electrode (BPE) was fabricated for the ultrasensitive electrochemiluminescence (ECL) detection of intracellular H₂O₂ and the number of cancer cells. In this approach, wax printing was used to fabricated reaction zone, and carbon ink-based BPE and driving electrodes were screen-printed into the paper. AuPd nanoparticles (NPs), which served as a carrier of the capture aptamer and as the catalyst for the ECL reaction of luminol and H₂O₂, were used to modify the BPE. Luminol/Au NPs were attached to the surface of the captured cells via hybridation chain reaction with two hairpin structure DNA labelled luminol/Au NPs. In the stimulation of phorbol myristate acetate, The coreactant H₂O₂ was released from the target cells. The ECL response of the luminol-H₂O₂ system was related to the number of cancer cells in the testing buffer, which served as a quantitative signal for the determination of cancer cells and the concentration of H₂O₂. In order to decrease the external voltage, K₃[Fe(CN)₆] was introduced in the cathode resevoir of BPE because it gained electrons at the cathode more easily than oxygen. The ECL intensity was quantitatively related to the concentration of MCF-7 in the range of 1.0 × 10²–1.0 × 10⁷ cells/mL. The detection limit was 40 cells/mL and it showed good specificity for cells with high overexpression of mucin-1 receptor, it was concluded that the developed protocol could be effectively utilized for the detection of MCF-7 cells.

在此手稿中，制造了一个由封闭双极电极（BPE）组成的一次性纸质分析设备，用于超灵敏电化学发光（ECL）检测细胞内H ₂ O ₂和癌细胞的数量。在这种方法中，将蜡印刷用于制造反应区，并将碳墨水基BPE和驱动电极丝网印刷到纸张中。AuPd纳米颗粒（NPs），用作捕获适体的载体和鲁米诺与H ₂ O ₂的ECL反应的催化剂用来修改BPE。通过与两个发夹结构DNA标记的鲁米诺/金纳米颗粒的杂交链反应，将鲁米诺/金纳米颗粒连接到捕获细胞的表面。在佛波醇肉豆蔻酸酯乙酸盐的刺激中，共反应剂H ₂ O ₂从靶细胞中释放出来。鲁米诺-H ₂ O ₂系统的ECL响应与测试缓冲液中癌细胞的数量有关，这是确定癌细胞和H ₂ O ₂浓度的定量信号。为了降低外部电压，K ₃ [Fe（CN）₆在BPE的阴极储液罐中加入了[]，因为它比氧气更容易在阴极获得电子。ECL强度与MCF-7浓度在1.0×10 ² –1.0×10 ⁷细胞/ mL范围内定量相关。检出限为40个细胞/ mL，对mucin-1受体高表达细胞显示出良好的特异性，得出的结论是所开发的方案可有效地用于MCF-7细胞的检测。