This manuscript describes a bioelectrochemical application of a new class of electrochemically generated hole-transporting (p-type) polymeric semiconductors (HTPS), which are based on a carbazole core and the oxiran and thiiran reactive groups. Electrode based on transparent layer of indium tin oxide was electrochemically modified with a layer of HTPS and a monolayer of covalently immobilized glucose oxidase (GOx). The HTPS/GOx-based electrode was investigated for an evaluation of direct hole-transfer between the enzyme and electrode at a bio-electrochemically relevant potential via HTPS layer. The broad linear relationship between the peak-current density and glucose concentration from 2 to 15 mM and high stability of ITO/poly-CzS/GOx-electrode was observed. Moreover, it was determined that charge transfer rate constants are reliable for the establishment of advanced electron transfer between enzyme and electrode for the application of this HTPS/GOx-based electrode in long-lived biofuel cells and amperometric biosensors.

该手稿描述了一种新型的电化学产生的空穴传输（p型）聚合物半导体（HTPS）的生物电化学应用，该聚合物基于咔唑核以及oxilan和thiiran反应基团。用一层HTPS和单层共价固定的葡萄糖氧化酶（GOx）对基于铟锡氧化物透明层的电极进行电化学修饰。研究了基于HTPS / GOx的电极，以评估通过HTPS层在生物电化学相关电位下酶和电极之间的直接空穴转移。观察到峰值电流密度与2至15 mM的葡萄糖浓度之间的宽线性关系以及ITO / poly-CzS / GOx电极的高稳定性。而且，