cAMP-responsive element-binding protein (CREB)-regulated transcriptional coactivator 3 (CRTC3) is well known to be related to obesity in humans and mice. However, the effects of CRTC3 have not been studied in pigs. Here, we characterized the structure of the porcine CRTC3 gene and identified single nucleotide polymorphisms (SNPs) in its coding region. Moreover, mRNA expression profiles of CRTC3 in muscle and fat tissues were examined. Of the 40 identified SNPs, the p.V515F mutation, located on exon 16, was genotyped in 368 Yorkshire pigs. The p.V515F mutation was significantly associated with lean meat production ability, including reduced back fat thickness (P = 0.0317) and loin eye area (P = 0.0174). Moreover, the SNP was significantly associated with differences in intermuscular fat (P = 0.0092), total muscle area in the belly (P = 0.0108), and total fat percentage in the belly (P = 0.0298). Taken together, our results suggest that the p.V515F mutation affects to lean meat production ability and amount of belly fat.

众所周知，cAMP反应元件结合蛋白（CREB）调控的转录共激活因子3（CRTC3）与人类和小鼠的肥胖有关。但是，尚未在猪中研究CRTC3的作用。在这里，我们表征了猪CRTC3基因的结构，并在其编码区确定了单核苷酸多态性（SNPs）。此外，检查了CRTC3在肌肉和脂肪组织中的mRNA表达谱。在鉴定出的40个SNP中，位于368号约克郡猪中的16号外显子存在p.V515F突变。p.V515F突变与瘦肉生产能力显着相关，包括降低的背脂肪厚度（P  = 0.0317）和腰眼面积（P = 0.0174）。此外，SNP与 肌肉间脂肪（P = 0.0092），腹部总肌肉面积（P  = 0.0108）和腹部总脂肪百分比（P  = 0.0298）的差异显着相关。两者合计，我们的结果表明p.V515F突变影响瘦肉的生产能力和腹部脂肪的数量。