Few-layered MoSe2 nanosheets as an efficient peroxidase nanozyme for highly sensitive colorimetric detection of H2O2 and xanthine†,Journal of Materials Chemistry B

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Few-layered MoSe2 nanosheets as an efficient peroxidase nanozyme for highly sensitive colorimetric detection of H2O2 and xanthine†
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-11-20 00:00:00 , DOI: 10.1039/c7tb02434g
Xiaoju Wu _{1,

2,

3,

4,

5} , Tongming Chen _{1,

2,

3,

4,

5} , Jianxing Wang _{1,

2,

3,

4,

5} , Guowei Yang _{1,

2,

3,

4,

5}

Affiliation

Molybdenum-containing and selenium-containing enzymes are generally prevalent in the biosphere, and the active sites of these involve molybdenum and selenium respectively. Herein, we for the first time demonstrated that few-layered molybdenum selenide (MoSe₂) nanosheets possess intrinsic peroxidase-like activity. The few-layered MoSe₂ nanosheets were prepared by a simple liquid exfoliation method. The catalytic process of the MoSe₂ nanosheets accords with the Michaelis–Menten behavior and they have a higher affinity to both TMB and H₂O₂ compared to HRP. In addition, we established that the MoSe₂ nanosheets catalyze the oxidation of TMB by promoting the electron transfer process from TMB to H₂O₂. More importantly, we applied the MoSe₂ nanosheets to the field of biological detection by developing a highly sensitive and selective colorimetric detection of H₂O₂ and xanthine concentration. With these advantages, the few-layered MoSe₂ nanosheets have critical potential in the diagnostics and biomedical fields.

中文翻译：

几层MoSe ₂纳米片可作为高效过氧化物酶纳米酶用于H ₂ O ₂和黄嘌呤的高灵敏比色检测†

含钼和含硒的酶通常在生物圈中普遍存在，其活性位点分别涉及钼和硒。在本文中，我们首次证明了很少层的硒化钼（MoSe ₂）纳米片具有固有的过氧化物酶样活性。通过简单的液体剥离方法制备了几层的MoSe ₂纳米片。MoSe ₂纳米片的催化过程符合Michaelis-Menten行为，并且与HRP相比，它们对TMB和H ₂ O _2的亲和力更高。此外，我们确定MoSe ₂纳米片通过促进电子从TMB转移到H ₂ O ₂来催化TMB的氧化。更重要的是，我们通过开发H ₂ O ₂和黄嘌呤浓度的高灵敏度和选择性比色检测技术，将MoSe ₂纳米片应用于生物检测领域。凭借这些优势，很少层的MoSe ₂纳米片在诊断和生物医学领域具有至关重要的潜力。

更新日期：2017-11-20

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