Hierarchical micro/submicrometer-scale structured scaffolds prepared via coaxial electrospinning for bone regeneration,Journal of Materials Chemistry B

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Hierarchical micro/submicrometer-scale structured scaffolds prepared via coaxial electrospinning for bone regeneration
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1039/c7tb02044a
Chen Tao _{1,

2,

3,

4} , Yanxia Zhang _{3,

4,

5,

6} , Bin Li _{1,

2,

3,

4,

7} , Liang Chen _{1,

2,

3,

4}

Affiliation

In this study, a cell-free bone tissue engineering scaffold based on core–sheath fibers with micro/submicro-scale structures were fabricated for bone regeneration. The composite fibers were prepared by coaxial electrospinning with cellulose acetate (CA) core solution and a sheath solution consisting of a mixture of silk fibroin (SF) and polyethylene oxide (PEO) loaded with nano-hydroxyapatite (nHAP) and bone morphogenetic protein 2 (BMP-2). The scanning electron microscopy results indicated that a submicro-scaled elliptical pattern formed throughout the entire sheath surface and that these patterned fibers formed a scaffold with microscale interconnected micropores. The fabricated scaffolds with micro/submicro-scale structures displayed good biocompatibility and increased mechanical properties compared to scaffolds based on single-component SF fibers without CA as the core. The results obtained with enzyme-linked immunosorbent assays (ELISAs) indicated that rapid initial release of BMP-2 from the scaffold occurs during the first few days, followed by slow and sustained release for as long as three weeks. The scaffold had a more profound effect on the attachment, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro compared to scaffolds without nHAP and BMP-2. Furthermore, in vivo studies indicated that this scaffold markedly enhanced bone regeneration at 12 weeks post-implantation. Taken together, our findings suggest that the hierarchical micro/submicro-scale structure scaffold consisting of core–sheath fibers acted as a good carrier for sustained BMP-2 release and that it can be used as a replacement material for bone grafts.

中文翻译：

通过同轴静电纺丝制备的用于骨骼再生的分级微米/亚微米级结构化支架

在这项研究中，制造了一种基于核-鞘纤维的无细胞骨组织工程支架，该支架具有微米/亚微米尺度的结构，用于骨骼再生。复合纤维是通过用醋酸纤维素（CA）核心溶液和由丝纤蛋白（SF）和负载有纳米羟基磷灰石（nHAP）和骨形态发生蛋白2的聚环氧乙烷（PEO）的混合物组成的皮鞘溶液进行同轴静电纺丝制备的BMP-2）。扫描电子显微镜结果表明，在整个鞘表面上形成了亚微米级的椭圆形图案，并且这些图案化的纤维形成了具有微米级互连微孔的支架。与基于无CA为核心的单组分SF纤维的脚手架相比，具有微米/亚微米级结构的制成的脚手架显示出良好的生物相容性和增强的机械性能。通过酶联免疫吸附测定（ELISA）获得的结果表明，BMP-2从支架中快速初始释放发生在头几天，然后缓慢而持续地释放长达三周。支架对骨髓间充质干细胞（BMSCs）的附着，增殖和成骨分化具有更深远的影响与不含nHAP和BMP-2的支架相比，在体外具有更高的耐受性。此外，体内研究表明，该支架在植入后12周时显着增强了骨再生。综上所述，我们的研究结果表明，由核心-皮层纤维组成的分级微/亚微米级结构支架可作为BMP-2持续释放的良好载体，并且可以用作骨移植物的替代材料。

更新日期：2017-11-20

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