Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease,Chemical Communications

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Detection of N6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease
Chemical Communications ( IF 4.3 ) Pub Date : 2017-11-13 00:00:00 , DOI: 10.1039/c7cc07699a
Miki Imanishi _{1,

2,

3,

4} , Shogo Tsuji _{1,

2,

3,

4} , Akiyo Suda _{1,

2,

3,

4} , Shiroh Futaki _{1,

2,

3,

4}

Affiliation

We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N⁶-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.

中文翻译：

基于MazF RNA核酸内切酶的甲基敏感性检测N ^6-甲基腺苷

我们发现大肠杆菌MazF毒素，一种ACA序列特异性内切核糖核酸酶，对N ^6-甲基腺苷（m6A）敏感，代表了第一个对m6A敏感的RNA裂解酶。MazF的甲基敏感性允许简单分析m6A脱甲基酶和甲基转移酶的活性。此外，该方法可用于抑制剂筛选。

更新日期：2017-11-20

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