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An engineered cell-imprinted substrate directs osteogenic differentiation in stem cells†
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-11-20 00:00:00 , DOI: 10.1039/c7bm00733g Khorshid Kamguyan 1, 2, 3, 4, 5 , Ali Asghar Katbab 1, 2, 3, 4 , Morteza Mahmoudi 3, 4, 6, 7, 8 , Esben Thormann 9, 10, 11, 12 , Saeed Zajforoushan Moghaddam 9, 10, 11, 12 , Lida Moradi 3, 4, 13, 14, 15 , Shahin Bonakdar 3, 4, 5, 16
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-11-20 00:00:00 , DOI: 10.1039/c7bm00733g Khorshid Kamguyan 1, 2, 3, 4, 5 , Ali Asghar Katbab 1, 2, 3, 4 , Morteza Mahmoudi 3, 4, 6, 7, 8 , Esben Thormann 9, 10, 11, 12 , Saeed Zajforoushan Moghaddam 9, 10, 11, 12 , Lida Moradi 3, 4, 13, 14, 15 , Shahin Bonakdar 3, 4, 5, 16
Affiliation
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A cell-imprinted poly(dimethylsiloxane)/hydroxyapatite nanocomposite substrate was fabricated to engage topographical, mechanical, and chemical signals to stimulate and boost stem cell osteogenic differentiation. The physicochemical properties of the fabricated substrates, with nanoscale resolution of osteoblast morphology, were probed using a wide range of techniques including scanning electron microscopy, atomic force microscopy, dynamic mechanical thermal analysis, and water contact angle measurements. The osteogenic differentiation capacity of the cultured stem cells on these substrates was probed by alizarin red staining, ALP activity, osteocalcin measurements, and gene expression analysis. The outcomes revealed that the concurrent roles of the surface patterns and viscoelastic properties of the substrate provide the capability of directing stem cell differentiation toward osteogenic phenotypes. Besides the physical and mechanical effects, we found that the chemical signaling of osteoinductive hydroxyapatite nanoparticles, embedded in the nanocomposite substrates, could further improve and optimize stem cell osteogenic differentiation.
中文翻译：
经过工程改造的细胞印迹基质可指导干细胞的成骨分化†
制作了一个细胞印迹的聚（二甲基硅氧烷）/羟基磷灰石纳米复合材料基材，以吸收形貌，机械和化学信号，以刺激和促进干细胞的成骨分化。使用广泛的技术（包括扫描电子显微镜，原子力显微镜，动态机械热分析和水接触角测量）来探查具有纳米级成骨细胞形态分辨率的人造基材的物理化学性质。通过茜素红染色，ALP活性，骨钙素测量和基因表达分析，探索了在这些底物上培养的干细胞的成骨分化能力。结果表明，表面图案和底物的粘弹性特性的同时作用提供了引导干细胞向成骨表型分化的能力。除了物理和机械作用外，我们发现嵌入纳米复合材料基质中的骨诱导性羟基磷灰石纳米颗粒的化学信号传导可以进一步改善和优化干细胞的成骨分化。
更新日期：2017-11-20
中文翻译：
经过工程改造的细胞印迹基质可指导干细胞的成骨分化†
制作了一个细胞印迹的聚（二甲基硅氧烷）/羟基磷灰石纳米复合材料基材，以吸收形貌，机械和化学信号，以刺激和促进干细胞的成骨分化。使用广泛的技术（包括扫描电子显微镜，原子力显微镜，动态机械热分析和水接触角测量）来探查具有纳米级成骨细胞形态分辨率的人造基材的物理化学性质。通过茜素红染色，ALP活性，骨钙素测量和基因表达分析，探索了在这些底物上培养的干细胞的成骨分化能力。结果表明，表面图案和底物的粘弹性特性的同时作用提供了引导干细胞向成骨表型分化的能力。除了物理和机械作用外，我们发现嵌入纳米复合材料基质中的骨诱导性羟基磷灰石纳米颗粒的化学信号传导可以进一步改善和优化干细胞的成骨分化。
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