A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229 kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC–MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1 → 6)-Gal (25.5%), (1 → 4)-Glc (18.3%), (1 → 6)-Glc (8.3%), (1 → 3)-Glc (5.3%) and (1 → 2)-Xyl (4.5%). GIPinv has branching points at (1 → 2, 6)-Man (8.6%) and (1 → 3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis. GIPinv should be further explored as a potential anti-cancer agent and a unique polysaccharide.

使用大小排阻和离子交换色谱法从不列颠哥伦比亚省发现的真菌的第四次氢氧化钠萃取步骤中纯化了抑制生长的多糖（GIPinv）。真菌在遗传上被鉴定为渐开线Paxillus复合体的成员。GIPinv的平均分子量为229  kDa，是由葡萄糖（65.9％），半乳糖（20.8％），甘露糖（7.8％），岩藻糖（3.2％）和木糖（2.3％）组成的杂聚糖。GC–MS甲基化分析表明，GIPinv在主链中具有混合键，包含（1  →  6）-Gal（25.5％），（1  →  4）-Glc（18.3％），（1  →  6）-Glc（8.3％） ，（1  →  3）-Glc（5.3％）和（1  →  2）-Xyl（4.5％）。GIPinv在（1 →  2，6）-Man（8.6％）和（1  →  3，6）-Man（4.9％）具有未取代的岩藻糖（8.3％）和葡萄糖（16.3％）作为末端糖。GIPinv对几种癌细胞具有生长抑制活性，并引发凋亡。GIPinv应该作为潜在的抗癌药和独特的多糖进行进一步研究。