The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay.

Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.

介绍了打算在口服免疫中应用的基于LBG的纳米颗粒的开发。纳米粒子的产生是通过温和的聚电解质络合而产生的，需要对LBG进行化学修饰。合成了三种LBG衍生物，即带正电的铵衍生物（LBGA）和带负电的硫酸盐（LBGS）和羧酸酯（LBGC）衍生物。这些通过傅里叶变换红外光谱，元素分析，核磁共振光谱，凝胶渗透色谱和X射线衍射进行表征。作为药物应用的目标，通过MTT试验和LDH释放试验对衍生物进行了毒理学分析。

使用LBGA或壳聚糖（CS）作为带正电荷的聚合物，以及LBGC或LBGS作为带负电荷的对应物，制备了几种纳米颗粒制剂，产生了具有足够特性的纳米颗粒，可用于口服免疫。