Aims Investigators have proposed that cardiovascular magnetic resonance (CMR) should have restrictions similar to those of ionizing imaging techniques. We aimed to investigate the acute effect of 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, and in a large cohort of patients in vivo. Methods and results In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, and histone H2AX phosphorylation (γ-H2AX) expression, leucocyte counts, and functional parameters were quantified using flow cytometry under the following conditions: (i) immediately following PBMC isolation, (ii) after standing on the benchside as a temperature and time control, (iii) after a standard CMR scan. In vivo study: blood samples were taken from 64 consecutive consenting patients immediately before and after a standard clinical scan. Samples were analysed for γ-H2AX expression and leucocyte counts. CMR was not associated with a significant change in γ-H2AX expression in vitro or in vivo, although there were significant inter-patient variations. In vitro cell integrity and function did not change with CMR. There was a significant reduction in circulating T cells in vivo following CMR. Conclusion 1.5 T CMR was not associated with DNA damage in vitro or in vivo. Histone H2AX phosphorylation expression varied markedly between individuals; therefore, small studies using γ-H2AX as a marker of DNA damage should be interpreted with caution. Cardiovascular magnetic resonance was not associated with loss of leucocyte viability or function in vitro. Cardiovascular magnetic resonance was associated with a statistically significant reduction in viable leucocytes in vivo.

中文翻译：

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1.5T心脏磁共振对人循环白细胞的影响

目的 研究人员提出心血管磁共振 (CMR) 应具有与电离成像技术类似的限制。我们旨在研究 1.5 T CMR 对白细胞 DNA 完整性、细胞计数和体外功能的急性影响，以及在体内的大量患者中。方法和结果体外研究：从健康志愿者中分离外周血单个核细胞（PBMCs），并在以下条件下使用流式细胞仪对组蛋白H2AX磷酸化（γ-H2AX）表达、白细胞计数和功能参数进行量化：（i） PBMC 隔离后，(ii) 站在工作台边作为温度和时间控制后，(iii) 在标准 CMR 扫描后。体内研究：在标准临床扫描之前和之后立即从 64 名连续同意的患者身上采集血样。分析样品的 γ-H2AX 表达和白细胞计数。尽管存在显着的患者间差异，但 CMR 与体外或体内 γ-H2AX 表达的显着变化无关。体外细胞完整性和功能不随 CMR 改变。CMR 后体内循环 T 细胞显着减少。结论 1.5 T CMR与体内外DNA损伤无关。组蛋白 H2AX 磷酸化表达在个体之间有显着差异；因此，应谨慎解释使用 γ-H2AX 作为 DNA 损伤标志物的小型研究。心血管磁共振与体外白细胞活力或功能的丧失无关。