Small post-translationally modified peptide hormones mediate crucial developmental and regulatory processes in plants. CLAVATA/ENDOSPERM-SURROUNDING REGION (CLE) genes are found throughout the plant kingdom and encode for 12–13 amino acid peptides that must often undergo post-translational proline hydroxylation and glycosylation withO-β1,2-triarabinose moieties before they become functional. Apart from a few recent examples, a detailed understanding of the structure and function of most CLE hormones is yet to be uncovered. This is mainly owing to difficulties in isolating mature homogeneously modified CLE peptides from natural plant sources. In this study, we describe the efficient synthesis of a synthetic Araf3Hyp glycosylamino acid building block that was used to access a hitherto uninvestigated CLE hormone from soybean called GmCLE40a. Through the development and implementation of a novelin vivoroot growth assay, we show that the synthetic triarabinosylated glycopeptide suppresses primary root growth in this important crop species.

中文翻译：

---

阿拉伯糖基化调节大豆肽激素CLE40a的生长调节活性。

小型翻译后修饰的肽激素介导植物中关键的发育和调控过程。整个植物界都发现了克拉瓦塔/胚乳周围区域（CLE）基因，它们编码12-13个氨基酸肽，这些肽必须经常经历翻译后脯氨酸的羟基化和O-β1,2-三阿拉伯糖部分的糖基化才能起作用。除了最近的一些例子外，尚未发现对大多数CLE激素的结构和功能的详细了解。这主要是由于难以从天然植物来源中分离成熟的均质修饰的CLE肽。在这项研究中，我们描述了合成的Araf3Hyp糖基氨基酸构件的有效合成，该构件用于从大豆中获得迄今未研究的CLE激素，称为GmCLE40a。