Recombinant Protein Polymers for Colloidal Stabilization and Improvement of Cellular Uptake of Diamond Nanosensors,Analytical Chemistry

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Recombinant Protein Polymers for Colloidal Stabilization and Improvement of Cellular Uptake of Diamond Nanosensors
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-11-17 00:00:00 , DOI: 10.1021/acs.analchem.7b03236
Tingting Zheng _{1,

2} , Felipe Perona Martínez ₃ , Ingeborg Maria Storm ₁ , Wolf Rombouts ₁ , Joris Sprakel ₁ , Romana Schirhagl ₃ , Renko de Vries ₁

Affiliation

Fluorescent nanodiamonds are gaining increasing attention as fluorescent labels in biology in view of the fact that they are essentially nontoxic, do not bleach, and can be used as nanoscale sensors for various physical and chemical properties. To fully realize the nanosensing potential of nanodiamonds in biological applications, two problems need to be addressed: their limited colloidal stability, especially in the presence of salts, and their limited ability to be taken up by cells. We show that the physical adsorption of a suitably designed recombinant polypeptide can address both the colloidal stability problem and the problem of the limited uptake of nanodiamonds by cells in a very straightforward way, while preserving both their spectroscopic properties and their excellent biocompatibility.

中文翻译：

重组蛋白聚合物用于胶体稳定和改善金刚石纳米传感器的细胞吸收。

鉴于荧光纳米金刚石本质上无毒，不漂白并且可以用作具有各种物理和化学特性的纳米级传感器这一事实，荧光纳米金刚石作为生物学中的荧光标记越来越受到关注。为了完全实现纳米金刚石在生物应用中的纳米传感潜力，需要解决两个问题：它们的有限的胶体稳定性，特别是在盐的存在下，以及它们被细胞吸收的能力有限。我们表明，适当设计的重组多肽的物理吸附可以以非常简单的方式解决胶体稳定性问题和细胞对纳米金刚石的有限吸收的问题，同时保留其光谱特性和出色的生物相容性。

更新日期：2017-11-19

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