Regulated transportation of nanoscale objects with a high degree of spatiotemporal precision is a prerequisite for the development of targeted molecular delivery. In vitro integration of the kinesin-microtubule motor system with synthetic molecules offers opportunities to develop controllable molecular shuttles for lab-on-a-chip applications. We attempted a combination of the kinesin-microtubule motor system with push–pull type azobenzene tethered inhibitory peptides (azo-peptides) through which reversible, spatiotemporal control over the kinesin motor activity was achieved locally by a single, visible wavelength. The fast thermal relaxation of the cis-isomers of azo-peptides offered us quick and complete resetting of the trans-state in the dark, circumventing the requirement of two distinct wavelengths for two-way switching of kinesin-driven microtubule motility. Herein, we report the manipulation of selected, single microtubule movement while keeping other microtubules at complete rest. The photoresponsive inhibitors discussed herein would help in realizing complex bionanodevices.

中文翻译：

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可见光有针对性地激活分子运输。

具有高度的时空精度的纳米级物体的规范运输是发展靶向分子传递的先决条件。驱动蛋白微管运动系统与合成分子的体外整合为开发可控制的分子梭用于芯片实验室应用提供了机会。我们尝试将驱动蛋白微管运动系统与推挽型偶氮苯系留的抑制肽（偶氮肽）相结合，通过单个可见波长局部实现对驱动蛋白运动活性的可逆时空控制。偶氮肽顺式异构体的快速热弛豫为我们提供了快速而完全的反式重置在黑暗中保持稳定状态，从而避免了驱动蛋白驱动的微管运动双向切换的两个不同波长的需求。在本文中，我们报告了选定的单个微管运动的操纵，同时使其他微管保持完全静止。本文讨论的光敏抑制剂将有助于实现复杂的仿生装置。